Acta Metallurgica Sinica
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鞠昊1a,宋雨来2,李志民1a,宋子琦 1a,冯婧 1a,公柏娟1b
基金资助:
科学技术部新型颌面软硬组织修复材料的研发(2016YFC1102800);中央高校基本科研业务费专项资金资助(JCKY-QKJC48)
Abstract:
Objective To study the in vitro biocompatibility of titannium alloy treated with micro-arc oxidation(MAO),in order to provide the theoretical basis for the next application of MAO in the treatment of titanium alloy implants. Methods The biofilm layer containing hydroxyapatite (HA) was formed on the surface of Ti6Al4V by one-step MAO (experiment group). The control groups were the untreated Ti6Al4V and the sandblasted Ti6Al4V. The three groups were co-cultured with osteoblasts to determine the proliferation,alkaline phosphatase (ALP) activity and mRNA expression of collagen Ⅰ (COL-Ⅰ) and osteocalcin (OC). Results The surface after the treatment was "crater" structure with a large number of HA. The in vitro results of three groups of materials showed that the cell proliferation rate of the Ti6Al4V treated with MAO was higher than that of the other two groups,and the ALP activity and COL-Ⅰand OC mRNA expression of cells were higher than those in the other two groups. Conclusion The biofilm layer formed on the surface of Ti6Al4V by MAO has a beneficial effect on the adhesion and proliferation of cells,which promotes the differentiation and shows good biocompatibility.
Key words: micro-arc oxidation;titanium alloy;hydroxyapatite, HA;biocompatibility
摘要:
目的 研究一步微弧氧化法处理钛合金(Ti6Al4V)的体外生物学性能,为下一步临床应用微弧氧化法处理钛合金种植体提供前期理论基础。方法 通过一步微弧氧化法在Ti6Al4V表面生成膜层并表征膜层形态(实验组),设置对照组为未处理的Ti6Al4V和喷砂酸蚀处理的Ti6Al4V,3组分别与成骨细胞共同培养,测定成骨细胞的增殖、碱性磷酸酶(alkaline phosphatase,ALP)活性以及Ⅰ型胶原蛋白(collagen Ⅰ,COL-Ⅰ)、骨钙素(osteocalcin,OC)的mRNA相对表达情况。结果 微弧氧化处理后Ti6Al4V表面含有大量的陶瓷烧结颗粒,以羟基磷灰石(hydroxyapatite,HA)为主要成分。3组试样的体外实验结果表明,经微弧氧化处理的Ti6Al4V表面的细胞增殖高于其他两组,细胞内的ALP活性、COL-Ⅰ、OC的mRNA相对表达也均高于其他两组。结论 通过微弧氧化法在Ti6Al4V表面生成的陶瓷膜对成骨细胞的增殖分化有促进作用,有良好的生物相容性。
关键词: 微弧氧化, 钛合金, 羟基磷灰石, 生物相容性
鞠昊1a,宋雨来2,李志民1a,宋子琦 1a,冯婧 1a,公柏娟1b . 一步微弧氧化法处理钛合金的体外生物相容性研究[J]. 中国实用口腔科杂志, DOI: 10.19538/j.kq.2018.06.009.
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URL: http://www.zgsyz.com/zgsykqk/EN/10.19538/j.kq.2018.06.009
http://www.zgsyz.com/zgsykqk/EN/Y2018/V11/I6/358