Serum S100B protein and GFAP detection in neonates born to mothers with preeclampsia and its clinical significance.

doi:10.7504/ek2014040114

Abstract

Abstract:

Abstract： Objective To discuss the changes of S100B protein and GFAP levels and its relationship with 1 min Apgar scoring in the serum of neonates born to mothers with preeclampsia of different degrees. Methods From Oct.2012 to Mar.2013 in Chilren’s hospital of Shanxi province ， 40 cases of newborns born to mothers with preeclampsia were divided into two groups: mild preeclampsia group （L group 20 cases）；severe preeclampsia group （H group 20 cases）；a healthy control group was established （N group 20 cases）；newborns of three groups were taken specimen at the time of admission ， dual- antibody sandwich enzyme-linked immunosorbent assay （ELISA） was used to detect the level of serum GFAP and S100B . In the first three days after admission cranial ultrasound was performed in each child. Results The difference among the L，H and N group was statistically significant （P2=17.20，P＜0.05）; S100B and GFAP levels in L and H group were positively correlated （γ= 0.658， P＜0.05）; S100B， GFAP levels in L and H group and neonatal 1min Apgar score were negatively correlated （γ$subScript$S100B$/subScript$=-0.482，γ$subScript$GFAP$/subScript$=-0.534，P＜0.01）. Conclusion The more serious eclampsia， the greater the possibility of brain damage， and the more serious the elevation of S100B and GFAP levels， and was negatively correlated with 1 min Apgar score， which indicates that the possibility of S100B protein and GFAP as a predictor of neonatal brain injury deserves further study.

Key words: Keywords： GFAP, S100B, preeclampsia, brain injury, neonate

摘要：

摘要：目的探讨S100B蛋白和胶质纤维酸性蛋白（GFAP）在不同程度子痫前期母亲所生新生儿血清中的变化、关系及与1 min Apgar评分的相关性，为早期预测新生儿脑损伤的可能性寻找依据。方法选择2012年10月至2013年3月在山西省儿童医院子痫前期母亲所生新生儿40例，分为2组：子痫前期轻度组（L组）20例、子痫前期重度组（H组）20例，设立健康对照组（N组） 20例，三组新生儿均于入院时留取标本，采用双抗体夹心酶联免疫吸附法（ELISA）法检测血清GFAP和S1OOB浓度。另每例患儿均于入院后第3天行头颅超声检查。结果 L组、H组、N组之间血清S100B、GFAP浓度比较差异有统计学意义（P2=17.20，P＜0.001）；L组、H组S100B与GFAP浓度呈正相关（γ=0.658，P＜0.05）；L组、H组S100B、GFAP浓度与新生儿1 min Apgar评分均呈负相关（γ$subScript$S100B$/subScript$=-0.482， γ$subScript$GFAP$/subScript$=-0.534，P＜0.01）。结论母亲子痫前期程度越严重，新生儿脑损伤可能性越大，S100B和GFAP浓度升高越明显，与1 min Apgar评分呈负相关，说明S100B蛋白和GFAP做为预测新生儿脑损伤的可能性值得进一步研究。

关键词: 关键词：胶质纤维酸性蛋白, S100B, 子痫前期, 脑损伤, 新生儿

SUN Jing,ZHANG Xiao-Li.. Serum S100B protein and GFAP detection in neonates born to mothers with preeclampsia and its clinical significance.[J]. Acta Metallurgica Sinica, DOI: 10.7504/ek2014040114.

孙晶,张小莉. 子痫前期母亲所生新生儿血清S100B蛋白和胶质纤维酸性蛋白检测及其临床意义[J]. 中国实用儿科杂志, DOI: 10.7504/ek2014040114.

References

［1］ Tanabe T，Okumura A，Komatsu M， et al. Clinical trial of minimal treatment for clustering seizures in cases of convulsions with mild gastroenteritis［J］. Brain Dev，2011，33（2）： 120-124.

［2］ Cusmai R，Jocic-Jakubi B，Cantonetti L， et al. Convulsions associated with gastroenteritis in the spectrum of benign focal epilepsies in infancy：30 cases including four cases with ictal EEG recording［J］. Epileptic Disord，2010，12（4）：255-261．

［3］ Schiff L，Hadker N，Weiser S， et al. A literature review of the feasibility of glial fibrillary acidic protein as a biomarker for stroke and traumatic brain injury［J］. Mol Diagn Ther，2012，16：79-92.

［4］ ZurekJ，Fedora M. Dynamics of glial fibrillary acidic protein during traumatic brain injury in children［J］. Trauma，2011，71：854-859．

［5］乐杰. 妇产科学［M］. 7 版. 北京：人民卫生出版社，2008： 92-101．

［6］ Pham N， Fazio V， Cucullo L， et al. Extracranial sources of S100B do not affect serum levels［J］. PLoS One，2010，5（9）. pii： e12691.

［7］ Stranjalis G， Korfias S， Psachoulia C， et al. The prognostic value of serum S-100B protein in spontaneous subarachnoid haemorrhage［J］. Acta Neurochir（Wien），2007，149（3）：231-238.

［8］ Unden J，Ingebrigtsen T，Romner B. Management of minor head injuries with the help of a blood test. S100B analysis can reduce the number of CT examinations and patient admissions ［J］. Lakartidningen，2008，105（24-25）：1846-1848.

［9］姜琴，刘兴莉，曾冬生，等.肠道病毒71型脑炎神经元特异性烯醇化酶S-100β变化及其临床意义分析[J]. 中国实用儿科杂志，2012，27（8）: 612-614.

［10］ Matsui T， Mori T， Tateishi N， et al. Astrocytic activation and delayed infarct expansion after permanent focal ischemia in rats. Part I. Enhanced astrocytic synthesis of S-100 beta in the periinfarct area precedes delayed infarct expansion［J］. Cereb Blood Flow Metab，2002，22（6）：711-722.

［11］ Gomes FC，Paulin D，Moura NV. Glial fibrillary acidic protein（GFAP）： modulation by growth factors and its implication in astrocyte differentiation［J］. Braz J Med Biol Res，1999，32（5）：619-631.

［12］范玉颖，刘波，王华，等. 新生期缺氧缺血性脑白质损伤大鼠模型比较研究[J].中国小儿急救医学，2013，20（2）:153-158.

［13］ Pang Y，Cai Z，Rhodes PG. Disturbance of oligodendrocyte development， hypomyelination and white matter injury in the neonatal rat brain after intracerebral injection of lipopolysacharide［J］.Brain Rer Dev Brain Rer，2003，140（2）：205-214.

［14］ Middeldorp J，Hol EM. GFAP in health and disease［J］. Prog Neurobiol，2011，93（3）： 421-443.

［15］周丛乐. 新生儿颅脑超声诊断学［M］. 北京：北京大学医学出版，2007：110-130.

［16］ Zhang H，Zhang Z，Zhang WW. Investigation of serum S-100B protein and neuron specific enolase of acute stroke patients［J］.Beijing Med J，2004，26（1）：28.

[1]	HUANG Shu-han，DANG Dan，FU Xin，et al. One case report of congenital thrombotic thrombocytopenic purpura [J]. CJPP, 2021, 36(5): 398-400.
[2]	ZHANG Ying. Advances in the application of pulmonary ultrasonography in neonates [J]. CJPP, 2020, 35(8): 655-659.
[3]	XIANG Ling-ling，SHAO Shi-qi，HUA Zi-yu. Impacts of evidence-based practice for improving clinical quality on clinical outcomes of premature in Department of Neonatology [J]. CJPP, 2020, 35(5): 391-396.
[4]	DING Li，SONG Wei，ZHU Xue-ping. Analysis of pathogen and high risk factors for poor prognosis in newborns with ventilator-associated pneumonia [J]. CJPP, 2020, 35(11): 877-880.
[5]	LIU Jing. Retrospect and prospect of lung ultrasound in Chinese children [J]. CJPP, 2019, 34(9): 753-756.
[6]	ZHANG Hai-qing*，CHEN Yan-ni. Contents and modes of the neonatal intensive rehabilitation [J]. CJPP, 2018, 33(8): 574-578.
[7]	CAO Jian-guo，YANG Xue. Rehabilitation of unintentional injuries and acute poisoning in children [J]. CJPP, 2018, 33(8): 592-595.
[8]	YAN Bei-bei, LI Xiao-ying. Research progress in intestinal microbiota of neonates and the related diseases [J]. CJPP, 2018, 33(7): 556-560.
[9]	WU Dong-xue，MA Jian-rong. Research progress in hypothermia treatment for hypoxic ischemic encephalopathy in neonates [J]. CJPP, 2018, 33(6): 463-467.
[10]	SHI Yuan*，ZHU Xing-wang. Clinical application of noninvasive high-frequency ventilation in preterm neonates [J]. CJPP, 2018, 33(5): 333-337.
[11]	GONG Hai-rong， CAO Yun，XU Hong. Early diagnosis of neonatal acute kidney injury in NICU [J]. CJPP, 2018, 33(2): 122-126.
[12]	GENG Lan-lan， GONG Si-tang. Difficulties and prospects of gastrointestinal endoscopy for neonates [J]. CJPP, 2018, 33(11): 866-869.
[13]	LUO Fang，DU Li-zhong. Application of amplitude-integrated EEG in neonatal ICU [J]. CJPP, 2017, 32(5): 328-332.
[14]	ZHANG Peng，ZHOU Wen-hao. Clinical application of therapeutic hypothermia in neonatal hypoxic-ischemic encephalopathy [J]. CJPP, 2017, 32(5): 332-336.
[15]	ZHANG Shu-cheng. Application of 24-hour gastric and esophageal double pH monitoring in neonatal gastroesophageal reflux diseases [J]. CJPP, 2017, 32(5): 337-341.