Abstract:

To detect the expression level of Zeta chain， and to probe its influence on the immunodeficiency and its role in children with PNS. Methods　Twenty-six children in first-onset，twenty-two children in relapse and thirty children in remission with PNS were studied， respectively.Twenty-one children were also studied as healthy control. The expression level of Zeta chain was examined by three-color flow-cytometry. Results　The mean fluorescence intensity（MFI） of Zeta chain in T and NK cell of peripheral blood was significantly reduced in first-onset and relapse as compared with healthy controls， respectively. （P < 0.05）， while an significant increase was observed during remission as compared with first-onset and relapse， respectively. （P < 0.05）. However， the proportion of T and NK cell of peripheral blood between groups was not significantly different （P > 0.05）. Between remission and healthy controls， the MFI of Zeta chain in T and NK cell of peripheral blood was not significantly difference （P > 0.05）. The MFI of Zeta chain in T and NK cell of peripheral blood was also not significantly difference between first-onset and relapse（P > 0.05）. Among these children with PNS， when nine children in first-onset and five children in relapse achieved remission， the expression level of Zeta chain was increased significantly（P < 0.05）， and there were two children in first-onset whose Zeta chain expression level was reduced again when relapse. Conclusions　The expression level of Zeta chain is significantly reduced in first-onset and relapse，and it is similar to that expressd in normal control in remission in children with PNS. Therefore， Zeta chain might be involved in the process of first-onset and relapse. The Zeta chain of peripheral blood might be one of markers which might be helpful to monitor disease condition and evaluate the effect of treatment in children with PNS

Key words: Zeta chain, nephrotic syndrome, T cell, NK cell, flow-cytometry

摘要：

检测原发性肾病综合征（PNS）患儿外周血T细胞和自然杀伤（NK）细胞中Zeta链的表达情况，探讨其对免疫紊乱的影响及其在儿童PNS中的作用和意义。方法　研究对象为上海交通大学附属儿童医院2007年11月至2010年5月住院和专科就诊的PNS患儿78例，分为初发组26例、复发组22例和缓解组30例，同时选取21名健康儿童作为对照组。用三色荧光标记流式细胞术检测外周血T和NK细胞中Zeta链的平均荧光强度（MFI）。结果　外周血T和NK细胞中Zeta链MFI在初发组和复发组较对照组明显降低，缓解组明显上升，差异均有统计学意义（P < 0.05）；各组间外周血T和NK细胞百分比差异均无统计学意义（P > 0.05）。缓解组与对照组、初发组与复发组间Zeta链MFI差异均无统计学意义（P均 > 0.05）。跟踪随访9例初发组和5例复发组患儿，其缓解时Zeta链水平明显上升，差异具有统计学意义（P < 0.05）；其中2例初发患儿缓解后复发时Zeta链水平再次下降。结论　Zeta链的表达在儿童PNS初发和复发中明显下降，缓解期恢复至正常水平。Zeta链可能参与了儿童PNS的初发和复发过程，或许可以作为监测病情和治疗评估的指标之一。

关键词: Zeta链, 肾病综合征, T细胞, 自然杀伤细胞, 流式细胞术