中国实用儿科杂志

中国实用儿科杂志 ›› 2022, Vol. 37 ›› Issue (2): 96-99.DOI: 10.19538/j.ek2022020604

• 专题笔谈 • 上一篇    下一篇

儿童肺炎病原体分子生物学检测技术的应用进展

  

  1. 昆明市儿童医院呼吸与危重症医学科  云南省儿童重大疾病研究重点实验室，云南  昆明  650034
  • 出版日期:2022-02-06 发布日期:2022-02-20
  • 通讯作者: 付红敏，电子信箱：510844660@qq.com
  • 基金资助:
    国家自然科学基金项目（81960021）

Application progress of molecular biological detection technology for pathogen of pneumonia in children

  1. Department of Pulmonary and Critical Care Medicine，Kunming Children’s Hospital；Yunnan Key Laboratory of Children’s Major Disease Research，Kunming  650034，China
  • Online:2022-02-06 Published:2022-02-20

摘要： 肺炎是儿童常见的呼吸系统感染性疾病，也是导致儿童死亡的重要原因。选择高效而准确的检测方法，尽快明确病原体，对肺炎的诊断和治疗十分关键。近几年来，分子生物学技术获得了快速发展，许多新的技术逐步应用于临床，如实时荧光定量PCR、生物芯片、二代测序、核酸等温扩增技术等，极大的提高了肺炎病原体的检测效率。本文对以上技术的优缺点进行了简要介绍。

关键词: 儿童, 肺炎, 病原体, 分子生物学技术, 应用

Abstract: Pneumonia is a common respiratory infectious disease and an important cause of death in children.Choosing efficient and accurate detection method to rapidly identify the pathogen is pivotal in diagnosis and treatment of pneumonia.In recent years，molecular biology technology has achieved rapid development，and many new technologies have been gradually applied in clinical practice，such as real-time fluorescent quantitative PCR，biochips，next-generation sequencing，nucleic acid isothermal amplification technology，etc.，which greatly improves the detection efficiency of pneumonia pathogens.This article briefly introduces the advantages and disadvantages of the above technologies.

Key words: child, pneumonia, pathogen, molecular biology technology, application