中国实用外科杂志 ›› 2011, Vol. 31 ›› Issue (05): 447-449.

• 临床研究生园地 • 上一篇    下一篇

乳腺癌微环境中调节性T细胞表达及其意义研究

严    丽1a,李清怀1a,李    莉2,蔡建辉1b   

  1. 1河北医科大学第二医院  a.腺体外科  b.胃肠外科,河北石家庄050000;2河北医科大学组织胚胎学教研室,河北石家庄050017
  • 出版日期:2011-05-01 发布日期:2011-05-30

  • Online:2011-05-01 Published:2011-05-30

摘要:

目的  探讨CD4+CD25+ Foxp3+调节性T细胞(Treg)在诱导乳腺癌免疫耐受中的作用和机制。方法  选取河北医科大学第二医院腺体外科乳腺癌标本40例(淋巴结无转移20例,转移20例)、乳腺纤维腺瘤标本20例,分别应用流式细胞术(FCM)和免疫组化检测CD4+CD25+Foxp3+ Treg、TGF-β和IL-10在乳腺癌原发灶、癌周组织、腋窝淋巴结以及乳腺纤维腺瘤中的表达。结果  乳腺癌原发灶中三指标表达程度,均较乳腺纤维腺瘤中高(P<0.05);原发灶中表达程度,均高于癌周组织和腋窝淋巴结(P<0.05);淋巴结转移组中癌周组织三项指标表达低于腋窝淋巴结(P<0.05),而淋巴结未转移组中,癌周组织和腋窝淋巴结表达无差异(P>0.05)。结论  CD4+CD25+Foxp3+Treg在乳腺癌微环境中富集可能是导致乳腺癌免疫逃逸的重要因素;CD4+CD25+Foxp3+Treg与TGF-β和IL-10具有一定的协同作用。

关键词: 乳腺癌;CD4+CD25+ Foxp3+调节性T细胞;TGF-&beta, ;IL-10

Abstract:

Research on expression and its role of regulatory T cells in mammary cancer microenvironment        YAN Li*, LI Qing-huai, LI Li, et al. *Department of Gland Surgery, the Second Hospital of Hebei Medical University, Shijiazhuang 050000, China
Corresponding author: CAI Jian-hui,E-mail:jianhuicai2001@163.com
Abstract    Objective    To investigate the role and mechanism of CD4+ CD25+ Foxp3+ regulatory T cells in immunity tolerance of mammary cancer. Methods    There were 40 cases of mammary cancer (20 cases of lymphonodus metastasis, 20 cases without lymphonodus metastasis) and 20 cases of mammary fibroma selected from the Department of Gland Surgery,the Second Hospital of Hebei Medical University. Expression level of CD4+ CD25+ Foxp3+ regulatory T cells, TGF-β and IL-10 in original, peripheral tissue, axilla lymphonodus and mammary fibroma of the above samples were measured respectively by FCM and immunohistochemical method. Results    The expression level of CD4+ CD25+ Foxp3+regulatory T cells, TGF-β and IL-10 in orginal tissues of mammary cancer were all higher than that of mammary fibroma (P<0.05), and were higher than that of peripheral tissue and axilla lymphonodus(P<0.05). In the group with lymphonodus metastasis, the expression level of the above three measured index in mammary cancer peripheral tissue was significantly lower than that expressed in axilla lymphonodus(P<0.05). However in the group without lymphonodus metastasis, there was no difference in expression level of the above three measured index between mammary cancer peripheral tissue and axilla lymphonodus (P>0.05). Conclusion    The fact that CD4+ CD25+ Foxp3+ regulatory T cells accumulate in mammary cancer microenvironment can probably result in immunity tolerance. With the progress of mammary cancer, there is some synergistic action between CD4+ CD25+ Foxp3+ regulatory T cells and TGF-β, IL-10.

Key words: mammary cancer, CD4+ CD25+ Foxp3+ regulatory T cells, TGF-β, IL-10