关键词: 循环肿瘤细胞, 胰腺导管腺癌, 计数, 病理学

Abstract:

Correlation between peripheral circulating tumor cells enumeration and clininical pathological characteristics of pancreatic ductal adenocarcinoma        XIN Bao-bao*，ZHOU Jia-ye， JI Yuan，et al. *Department of Pancreatic Surgery，Zhongshan Hospital，Fudan University，Shanghai 200032，China
Corresponding author：LOU Wen-hui，E-mail:lou.wenhui@zs-hospital.sh.cn
Abstract    Objective    To study whether the theory of circulating tumor cells is practical and observe its clinical value according to the comparison with pathological data. Methods    A total of 22 patients who was suspected with pancreatic ductal adenocarcinoma was recruited consecutively from October 2014 to June 2015 in Zhongshan Hospital of Fudan University. The peripheral blood sample was obtained post-resection. Among them, there were 14 males and 8 females and the mean age was (63.91±9.31) years old. Each patients was drawn 7.5mL of blood from peripheral vein. The platform of CellSearch was used to capture the tumor cells. The correlations between the tumor cell enumeration and the pathological data were analyzed. Results    All the patients were perfomred radical resection. No chemotherapy was performed before surgery. Overall, EpCAM (+)-CTCs were captured in 15 patients (15/22, 68.18%), and the enumeration range was 0—13/7.5mL peripheral blood. Compared with the control group, patients with lymph metastases, tumor size larger than 2 cm accompanied with CA19-9 elevated preoperatively were likely to have higher capture rate of tumor cells（P=0.014）. Conclusion    Peripheral circulating tumor cells enumeration is practical in pancreatic ductal adenocarcinoma, which plays certain role in predicting the prognosis of those patients.

Key words: circulating tumor cell, pancreatic ductal adenocarcinoma, enumeration, pathology