Abstract:

Abstract： Objective To explore effects of different dosage of diclofenac sodium combined with lipopolysaccharide （LPS） on expression of IL-1βand TNF-αin human periodontal ligament fibroblasts（HPDLFs）. Methods Take cryopreserved HPDLFs to culture. After cell morphology identification and being induced by 10 μg/mL of LPS， the HPDLFs were intervened respectively by 0.1，1， 10， 50 and 100mg/L of diclofenac sodium. Then the changes of the expression levels of IL-1βand TNF-α in cultured HPDLFs were observed by ELISA method. Results Diclofenac sodium was able to down-regulate the expression level of IL-1βand TNF-α in cultured HPDLFs which was induced by the same concentration of LPS. And the gradually decreasing trend of IL-1β and TNF-α expression was obviously related to the increasing diclofenac sodium dosage （P＜0.05）. Conclusion Diclofenac sodium may play an important role in inhibiting the expression of IL-1βand TNF-α in LPS-induced HPDLFs， which provides a new way of the clinical treatment for periodontal diseases.

Key words: diclofenac sodium；lipopolysaccharide；human periodontal ligament fibroblasts；IL-1&beta, ；TNF-&alpha

摘要：

目的 探讨双氯芬酸钠对脂多糖（LPS）诱导的人牙周膜成纤维细胞（HPDLFs）白细胞介素-1β（IL-1β）和肿瘤坏死因子-α（TNF-α）表达的影响。方法 本研究于2013年3—5月在山西医科大学口腔实验室进行。将复苏培养冻存的HPDLFs进行形态学鉴别后，用10 μg/mL的LPS进行诱导，分别以0.1、1、10、50、100 mg/L的双氯芬酸钠进行干预，酶联免疫吸附试验法（ELISA法）检测HPDLFs表达IL-1β和TNF-α水平的变化。结果 对同一质量浓度LPS诱导的HPDLFs而言，双氯芬酸钠能下调HPDLFs表达IL-1β和TNF-α的水平，并且随着双氯芬酸钠质量浓度的增加，IL-1β和TNF-α的表达量呈逐渐减弱的趋势（P＜0.05）。结论 双氯芬酸钠可能对于LPS诱导的HPDLFs IL-1β和TNF-α的表达有重要的抑制作用，这为牙周病的临床治疗提供了新的思路。

关键词: 双氯芬酸钠, 脂多糖, 人牙周膜成纤维细胞, IL-1&beta, TNF-&alpha