Abstract:

Abstract：Objective To investigate the effect of glucocorticoids on periodontal ligament cell （PDLC） expressing osteoprotegerin. Methods Healthy premolars extracted for orthodontic reasons from a 12- year- old boy were obtained， and periodontal tissues were collected and cultured to obtain PDLCs. PDLCs were obtained by means of limited dilutions and were characterized as positive cloned cells. Dexamethasone （DEX） was added into culture media and their effects on PDLC proliferation and OPG secretion were observed. The OPG concentrations in cell culture supernatants were detected by sandwich ELISA. Living cell numbers were demonstrated by MTT result. Results DEX above 40 ng/mL decreased the opg and opg/mtt values （P＜0.001） but had no effect on mtt values （P＞0.05）. Conclusion Glucocorticoids might increase periodontal bone resorption by inhibition of OPG production in periodontal ligament cells.

Key words: periodontium, cell culture, glucocorticoids

摘要：

目的 研究糖皮质激素（glucocorticoids）对牙周膜细胞表达骨保护因子（osteoprotegerin， OPG）的影响。方法 临床收集青少年患者的新鲜正畸拔除前磨牙，刮取牙周膜组织进行细胞培养。采用有限稀释法获得牙周膜细胞克隆，并进行细胞克隆鉴定。培养基中加入地塞米松（dexamethasone， DEX），MTT法检测牙周膜细胞增殖水平，夹心酶联免疫吸附法测定培养上清液中OPG含量。结果 DEX质量浓度在40 ng/mL以上时显著降低细胞对OPG的表达量（P＜0.001）；DEX对细胞增殖没有明显影响（P＞0.05）。结论 糖皮质激素可以通过抑制牙周膜细胞对OPG的表达提高牙周骨质吸收活性。

关键词: 牙周膜, 细胞培养, 糖皮质激素