Abstract:

Abstract：Objective To discuss the effect of Hedyotis Difusa Injection（HDI） on tongue squamous cell carcinoma TCA8113 and its effect on RAS/RAF signal pathway factor. Methods MTT was used to detect the survival rate in tongue squamous cell carcinoma TCA8113 after being affected by different concentrations of HDI，and detect the expression and variation of the key RAS/RAF signal pathway factor protein by using western blot detection method. Results After the HDI intervening the tongue squamous cell carcinoma，MTT showed that HDI could restrain the general proliferation of TCA8113 observably and had a concentration reliance（P＜0.05）.The key RAS/RAF signal pathway factor expression was concentration-dependent. Conclusion HDI can restrain the general proliferation of tongue squamous cell carcinoma TCA8113 and the signal transition of the RAS/RAF path way，thus plays its antineoplastic role.

Key words: Hedyotis Difusa injection, tongue squamous cell carcinoma, RAS/RAF pathway

摘要：

目的 探讨白花蛇舌草注射液（HDI）对口腔舌鳞癌TCA8113细胞增殖及RAS/RAF通路信号传导的影响。方法 本研究于2013年3月在佳木斯大学口腔医学实验中心进行。使用四甲基偶氮唑蓝（MTT）方法测定不同浓度HDI对舌鳞癌TCA8113作用后的存活率情况，并用使用免疫蛋白印迹（Western blot）方法检测RAS/RAF信号通路关键蛋白RAS、RAF的表达和变化。结果 HDI干预舌鳞癌TCA8113细胞后，MTT显示HDI可明显抑制其细胞增殖，具有浓度依赖（P＜0.05）。当HDI浓度为30%时，抑制率为94.7%。RAS/RAF信号通路关键蛋白RAS、RAF的表达呈浓度依赖性下调。结论 HDI可抑制舌鳞癌细胞TCA8113细胞增殖及抑制RAS/RAF通路信号传导，发挥抗肿瘤作用。

关键词: 白花蛇舌草注射液, 舌鳞癌细胞, RAS/RAF信号通路