Abstract:

Objective    To investigate the expression of macrophage migration inhibitory factor（MIF）in the serum and gingival tissues of patients with chronic periodontitis，and to explore its role in clinical prognosis. Methods    Serum samples were collected from chronic periodontitis patients（18 cases）and healthy controls（18 cases），MIF and monocyte chemotactic protein-1（MCP-1）levels were assayed by enzyme-linked immunosorbent assay. Gingival tissues samples were collected from chronic periodontitis patients（before and after periodontal initial therapy）and periodontally healthy individuals. Then the MIF and MCP-1 expression were detected with immunohistochemical method. The data was analyzed with SPSS 24.0 software package. Results     Serum MIF and MCP-1 were significantly higher in periodontitis patients compared with healthy controls（P < 0.05）. MIF protein was expressed in gingival epithelia，and the MIF level was highest in periodontitis gingiva；its level was decreased by periodontal initial therapy，but still higher than the expression of MIF in healthy gingiva （P < 0.05）；MCP-1 protein was also expressed in both gingival epithelia and connective tissues，but there was no significant difference between the periodontitis patients and healthy controls（P > 0.05）. Conclusion          MIF is involved in the progression of chronic periodontitis as an inflammatory factor，and its level may be used as auxiliary indicators for the evaluation of the activity and degree of periodontitis.

Key words: chronic periodontitis, macrophage migration inhibitory factor, monocyte chemotactic protein-1

摘要：

目的    观察并分析慢性牙周炎患者血清及牙龈组织中巨噬细胞移动抑制因子（macrophage migration inhibitory factor，MIF）表达及其临床意义。方法    选择2016年6月至2017年6月就诊于中国医科大学附属口腔医院牙周科及口腔外科的患者，收集重度慢性牙周炎患者（牙周炎组，18例）及牙周健康者（健康对照组，18例）血清，酶联免疫吸附试验检测MIF及单核细胞趋化蛋白-1（monocyte chemotactic protein-1，MCP-1）水平。收集未经治疗（CP组，8例）及牙周基础治疗后1个月（SRP组，11例）的重度慢性牙周炎患者、牙周健康者（H组，18例）的牙龈组织，采用免疫组化法检测MIF及MCP-1在牙龈中的表达。采用SPSS 24.0软件t检验比较血清中MIF及MCP-1表达的差异，单因素方差分析比较CP组、SRP组及H组牙龈组织中MIF及MCP-1表达的差异。结果    慢性牙周炎患者血清中MIF及MCP-1浓度较牙周健康者明显升高（P < 0.05）。MIF在牙龈上皮呈阳性表达，且在未经治疗的CP组表达水平最高，经牙周基础治疗后的SRP组MIF表达水平显著下降，但仍高于H组（P < 0.05）；MCP-1在牙龈组织的上皮层和结缔组织中均有弱表达，表达水平在3组间差异无统计学意义（P > 0.05）。结论    MIF作为一种炎症因子参与慢性牙周炎的发展进程，其表达水平可作为炎症指标辅助慢性牙周炎活动性及炎症程度的判断。

关键词: 慢性牙周炎, 巨噬细胞移动抑制因子, 单核细胞趋化蛋白-1