Abstract:

Objective    To discuss the influence of glycogen synthase kinase-3β（GSK-3β）on osteogenesis of jaw bone marrow mesenchymal stem cells（JBMMSCs） in inflammatory microenvironment. Methods    Use shRNA to knock down the expression of GSK-3β in JBMMSCs. IL-1β and TNF-α were used to mimic inflammatory microenvironment. After osteogenesis，Western Blot was used to detect osteogenesis-related protein expression and Alizarin Staining was used to detect the efficiency of osteogenesis in different groups. Results    Expression of osteogenesis-related protein and efficiency of osteogenesis was highly up-regulated after knocking-down of GSK-3β in JBMMSCs. Inflammatory microenvironment could significantly down-regulate the expression of osteogenesis-related protein and efficiency of osteogenesis in ordinary JBMMSCs，while knocking-down GSK-3β could attenuate this effect. Conclusion    Knocking-down of GSK-3β in JBMMSCs can both increase the efficiency of osteogenesis and resist the adverse effect of inflammatory microenvironment on osteogenesis，thus GSK-3β is a potential target for improving the regeneration in inflammatory microenvironment.

Key words: glycogen synthase kinase-3&beta, ；inflammatory microenvironment；osteogenesis

摘要：

目的    探讨炎性微环境中糖原合成酶激酶-3β（GSK-3β）对颌骨来源间充质干细胞（JBMMSCs）成骨分化的影响。方法    应用慢病毒（shRNA）转染技术在JBMMSCs中敲减GSK-3β表达，利用白细胞介素（IL）-1β和肿瘤坏死因子（TNF）-α模拟炎症微环境。成骨诱导后利用Western Blot检测成骨相关蛋白表达，茜素红染色检测成骨效率。结果    敲减GSK-3β表达可增强JBMMSCs成骨诱导后的成骨相关蛋白表达，成骨效率增强。炎症微环境可显著下调正常JBMMSCs成骨诱导后的成骨相关蛋白表达，成骨效率明显降低，而对敲减GSK-3β表达的JBMMSCs成骨诱导后的成骨相关蛋白表达下调较弱，成骨效率降低较轻。结论    抑制GSK-3β既可促进JBMMSCs的成骨作用，又可减轻炎性微环境对JBMMSCs成骨的不良影响，为提高炎症状态下JBMMSCs促进组织再生提供了新的治疗靶点。

关键词: 糖原合成酶激酶-3&beta, 炎性微环境, 成骨诱导