中国实用口腔科杂志

中国实用口腔科杂志

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自制含钛镍铬烤瓷合金体外细胞毒性实验

康晓燕1，战德松2   

  1. 作者单位：1.沈阳市口腔医院修复科，沈阳110002；2.中国医科大学附属口腔医院干诊科，沈阳110001
  • 出版日期:2013-11-15 发布日期:2013-11-26
  • 通讯作者: 战德松

Cytotoxicity experiment in vitro of two kinds of self-made nickel-chromium ceramic alloy containing titanium.       

KANG Xiao-Yan**，ZHAN De-song.   

  1. *Department of Prosthetics，the Stomatological Hospital of  Shenyang， Shenyang 110002，China
  • Online:2013-11-15 Published:2013-11-26

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摘要：

目的 评价2种自制含钛镍铬烤瓷合金的细胞毒性。方法 本研究于2009年5—12月在中国医科大学中心实验室完成。分别将2种自制含钛镍铬烤瓷合金（2、3号合金）、纯钛、普通镍铬合金及铅的浸提液与体外培养的细胞接触后，进行MTT实验来评定材料的细胞毒性。结果 2、3号合金毒性为1级，钛的毒性为0级，镍铬毒性为1级，铅在细胞培养72 h后毒性级由1级增至2级。结论 自制的含钛镍铬烤瓷合金符合临床应用要求，生物相容性好于目前临床常用的普通镍铬合金。

关键词: 镍铬烤瓷合金 , 细胞毒性, MTT法

Abstract:

Abstract：Objective To test the cytotoxicity of two kinds of self-made nickel-chromium ceramic alloy containing titanium. Methods From May 2009 to Dec. 2009 in the Centric Lab. of China Medical University the test was performed. After mixing the maceration extraction of the materials with the cultures of L-929 cells，we evaluated cytotoxicity by using tetrazolium-based colorimetric assay. Results The cytotoxicity of the two kinds of self-made nickel-chromium ceramic alloy was stronger than that of pure Ti，and weaker than that of Ni-Cr alloy. Conclusion The self-made nickel-chromium ceramic alloy containing titanium meet the clinical requirement and has better biocompatibility than the currently clinically commonly used alloy.

Key words: nickel-chromium ceramic alloy, cytotoxicity, MTT-assay

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