中国实用口腔科杂志

中国实用口腔科杂志 ›› 2011, Vol. 4 ›› Issue (01): 28-30.

• 论著 • 上一篇    下一篇

辛伐他汀对糖尿病大鼠种植体周围骨组织中骨形态发生蛋白-2表达影响的研究

  

  1. 山西医科大学口腔医院，太原  030001
  • 收稿日期:2010-11-25 修回日期:2010-12-30 出版日期:2011-01-15 发布日期:2011-02-22

  • Received:2010-11-25 Revised:2010-12-30 Online:2011-01-15 Published:2011-02-22

摘要：

目的    探讨辛伐他汀对糖尿病大鼠种植体周围骨组织中骨形态发生蛋白-2（BMP-2）表达的影响及临床意义。方法    2010年5—8月在山西医科大学取成年雄性SD大鼠36只，适应性喂养1周无异常后，随机分为A、B1、B2 3组，每组12只。A组予腹腔内注射柠檬酸盐缓冲液，B1、B2组按50 mg／kg体重剂量予腹腔内注射链尿佐菌素（STZ），建立糖尿病大鼠模型。成模后，在3组大鼠胫骨近骺端种植纯钛种植体。B2组大鼠每日予辛伐他汀5 mg／kg体重灌服，A、B1两组大鼠每只每日灌服生理盐水1.5 mL。灌胃12周后，处死动物，采用免疫组化方法检测种植体周围骨组织中BMP-2的表达。结果    B2组BMP-2阳性细胞比例明显高于B1组 ，差异有统计学意义（P < 0.01）。结论    辛伐他汀能使糖尿病大鼠骨组织 BMP-2 表达增强，改善骨代谢，有促进种植体骨结合的作用。

关键词: 辛伐他汀, 糖尿病, 种植体, 骨形态发生蛋白-2

Abstract:

Objective    To investigate the expression and clinical significance of bone morphogenetic protein 2（BMP-2） in bone tissue around the implant after simvastatin intervention.  Methods    After 1-week adaptive feeding without abnormal condition， 36 female adult SD rats were equally divided into A group （normal control group）and B group （experimental group）. Group A was intraperitoneally injected with citrate buffer. Group B was intraperitoneally injected with streptozotocin （STZ） 50mg／kg to set up the diabetic rats model.After the model was successfully established， A group and B group were implanted with Titanium in the proximal tibial metaphysic.B group was randomly allocated to B1 group （Diabetes Mellitus+ implant group） and B2 group （Diabetes Mellitus+ implant + simvastatin group）.There were 12 rats in each group. B2 rats were given orally simvastatin 5mg／（d·kg）；A and B1 rats were given orally normal saline 1.5mL／d. After 12 weeks of feeding the rats were sacrificed. Immunohistochemical technique was used to assess the expression of BMP-2 in bone tissue around the implant. Results    Positive rate of cells in B2 group was significantly higher than that in B1 group. The difference was statistically significant（P < 0.01）. Conclusion    Simvastatin can enhance the expression of BMP-2 gene in bone tissue， improve bone metabolism and implant osseointegration.

Key words: simvastatin；diabetes mellitus； implant； BMP-2