中国实用口腔科杂志

中国实用口腔科杂志 ›› 2010, Vol. 3 ›› Issue (09): 543-544.

• 论著 • 上一篇    下一篇

蛋白激酶B参与正畸牙周组织改建作用实验研究

  

  1. 中国医科大学口腔医学院奉天门诊，辽宁省口腔医学研究所，沈阳  110013
  • 收稿日期:2010-06-25 修回日期:2010-08-15 出版日期:2010-09-15 发布日期:2010-10-14

  • Received:2010-06-25 Revised:2010-08-15 Online:2010-09-15 Published:2010-10-14

摘要：

目的    探讨正畸力作用下蛋白激酶B（Akt）在兔牙周组织改建过程中的作用。方法    2009年1—4月在中国医科大学中心实验室选取日本大耳白兔24只，建立正畸牙移动动物模型，将实验动物上颌右侧戴矫治器，作为实验侧；左侧未戴矫治器，作为对照侧。分别在戴矫治器后3、5、7、14 d各处死6只实验动物。用实时荧光定量聚合酶链反应（RQ-PCR）方法对牙周组织中Akt表达进行检测。结果    RQ-PCR检测结果显示，加力3 d后牙周组织中Akt mRNA表达增强（P < 0.05），7 d后牙周组织中Akt mRNA明显增强（P < 0.01），随后缓慢下降。与对照侧相比，实验侧牙周组织中Akt mRNA表达明显增强（P < 0.05）。结论    Akt参与牙周组织改建，并在牙周组织改建中起重要作用。

关键词: Akt, 正畸力, 实时荧光定量PCR

Abstract:

Objective    To study the role of Akt in periodontal tissues remodeling during orthodontic tooth movement.  Methods    Twenty-four rabbits were chosen to establish rabbit models for the study from January to April in the central laboratory of China Medical University. The right maxillary teeth of each animal treated by orthodontics were the test sides，and the untreated left teeth were the control sides. The animals were sacrificed at 3，5，7，14 d，respectively. The prepared tissue specimens were processed for the study of the expression of Akt in periodontal tissues by Real-time quantitative PCR. Results    Real-time quantitative PCR showed that the expression of Akt mRNA dramatically changed at 3 d. The expression of Akt mRNA in treating periodontal tissues was higher than the control side，especially at 7 d，and then decreased. Compared with the control side，there was significant difference in statistical analysis（P < 0.05）. Conclusion     Higher expression of Akt during orthodontic tooth movement plays an important role in the process of periodontium remodeling.

Key words: Akt；orthodontics；real-time fluorescence quantitative PCR