中国实用口腔科杂志

中国实用口腔科杂志 ›› 2010, Vol. 3 ›› Issue (09): 538-539.

• 论著 • 上一篇    下一篇

滋阴活血方药对糖尿病大鼠牙周组织保护作用研究

  

  1. 河北医科大学口腔医学院，石家庄  050017
  • 收稿日期:2010-07-13 修回日期:2010-08-25 出版日期:2010-09-15 发布日期:2010-10-14

  • Received:2010-07-13 Revised:2010-08-25 Online:2010-09-15 Published:2010-10-14

摘要：

目的    探讨滋阴活血方药（nourishing yin and promoting blood-flow recipe，NYPBR）对糖尿病大鼠牙周组织的保护作用。方法    2007年6—10月于河北医科大学选用6月龄雄性Wistar大鼠28只，随机分为 3组：空白对照组（8只）、糖尿病组（10只）和中药治疗组（10只）。除空白对照组外，其余各组大鼠腹腔内注射链脲佐菌素（STZ）溶液，建立糖尿病大鼠模型。模型建立后，中药治疗组灌胃给予NYPBR 12周，结束给药后第1天处死大鼠，取上颌骨标本苏木素-伊红（HE）染色，光镜观察牙周组织病理变化。结果    糖尿病组大鼠牙龈上皮和固有层中度炎细胞浸润，毛细血管数目增多、扩张充血，纤维排列紊乱、断裂，骨吸收陷窝多，陷窝内见破骨细胞，牙槽嵴顶无吸收；而中药治疗组牙周组织结构与空白对照组相似。结论    NYPBR能显著减轻糖尿病造成的牙周组织损伤。

关键词: 糖尿病, 牙周组织, 滋阴活血方药

Abstract:

Objective    To explore protective effect of Nourishing Yin and Promoting Blood-flow Recipe（NYPBR） on periodontal tissue of diabetes rats. Methods    During the time between June and October 2007， 28 six-month-old male Wistar rats were randomly divided into three groups in Hebei Medical University ： 8 in the control group， 10 in both the DM group and the NYPBR group. The DM model was made and NYPBR was administered for every rat in the NYPBR  group. After 12 weeks of medicine administration， the rats were killed and the maxilla were collected. The tissue sections were stained with HE for histological examination. Results    In the DM group， inflammatory cells infiltration in gingiva epithelium and lamina propria were observed. The number of blood capillary， whose vessel lumen dilated and hyperemic， increased. Collagen fibrils were in disorder and fractured. Osteoclasts were found in the increased osseous lacunas under the alveolar bone. However， there was no absorption on alveolar ridge crest. In the NYPBR  group， the structure of periodontal tissue was similar to that of the control group. Conclusions    NYPBR can effectively alleviate injury of periodontal tissue caused by DM.

Key words: diabetes；periodontal tisssues；NYPBR