中国实用口腔科杂志

中国实用口腔科杂志

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伴放线放线杆菌粗糙株外膜蛋白的电泳分析

王 楠，钟德钰，张 晶，徐全臣   

  1. 青岛大学医学院附属医院口腔科
  • 收稿日期:2009-09-23 修回日期:2009-10-20 出版日期:2009-11-15 发布日期:2010-04-02

  • Received:2009-09-23 Revised:2009-10-20 Online:2009-11-15 Published:2010-04-02

摘要：

目的 分析不同血清型伴放线放线杆菌粗糙型菌株外膜蛋白的差异。方法 于2009年8—9月，在青岛大学医学院选用3种不同血清型的伴放线放线杆菌粗糙型菌株D7S、SA716和SA1151，分别培养并收集菌细胞，采用超声破碎及超离心技术提取外膜蛋白，SDS-PAGE电泳方法分析其分子质量。结果 全细胞电泳显示，3个菌株蛋白带的分子质量在10～200 ku之间分布，3个菌株之间蛋白带分布未见明显差异;提取的外膜蛋白电泳显示，3个菌株有共同的6个蛋白带，分子质量分别为100、75、64、45、34、31 ku;SA1151和SA716存在共同的分子质量为29 ku的蛋白带;D7S和SA716存在共同的分子质量为27 ku的蛋白带;SA1151存在清晰的分子质量为110 ku的蛋白带。结论 不同血清型伴放线放线杆菌粗糙型菌株之间的外膜蛋白可能存在一定的差异。

关键词: 伴放线放线杆菌, 外膜蛋白, SDS-PAGE电泳

Abstract:

Objective To separate and analyse outer membrane proteins from Aggregatibacter actinomycetemcomitans rough strains of different serotypes and identify them by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Methods Three kinds of different serotype of Aggregatibacter actinomycetemcomitans rough strains（D7S、SA716、SA1151） were cultured. Their cells were collected and their outer membrane proteins were extracted by super centrifugation and sonicate. The extracts were identified by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Results There was no significant difference in the whole cell electrophoresis of three strains; six common outer membrane proteins from three strains were identified and they were 100 Ku、75 Ku、64 Ku、45 Ku、34 Ku、31 Ku. The protein of 29 Ku was found in SA716 and SA1151， the protein of 27 Ku was found in SA716 and D7S， and the protein of 110 Ku was only found in SA1151. Conclusion There may be some differences in the outer membrane proteins of different serotype Aggregatibacter actinomycetemcomitans rough strains.

Key words: Aggregatibacter actinomycetemcomitans, outer membrane proteins（Omp）, SDS-PAGE electrophoresis