中国实用口腔科杂志

中国实用口腔科杂志

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4种中药体外抗口腔念珠菌的敏感性研究

郑童娇，张 虹   

  1. 大连医科大学附属第一医院口腔科
  • 收稿日期:2008-02-27 修回日期:2008-09-04 出版日期:2008-09-09 发布日期:2008-09-09

  • Received:2008-02-27 Revised:2008-09-04 Online:2008-09-09 Published:2008-09-09

摘要：

目的 测定丁香酚、柠檬醛、肉桂醛、八角茴香油的体外抗口腔念珠菌活性，为抗口腔念珠菌中药的进一步开发提供实验依据。方法 收集2006年1 — 10月大连医科大学附属第一医院口腔科门诊38例口腔念珠菌感染患者。参照美国国家临床试验标准委员会（NCCLS）标准，用微量液基稀释法分别检测丁香酚、柠檬醛、肉桂醛和八角茴香油对38株口腔念珠菌临床分离株的最小抑菌浓度（MIC），并以氟康唑为质控药物，ATCC 6258和ATCC 90028为质控株控制整个实验条件。结果 4种药物对白色念珠菌和克柔念珠菌的MIC分别为：丁香酚199.50 ~ 399.00 mg／L和399.00 mg／L;柠檬醛166.31 ~ 332.63 mg／L和166.31 mg／L;肉桂醛23.55 ~ 94.20 mg／L和47.10 mg／L;八角茴香油1218.75 ~ 2437.50 mg／L和1218.75 mg／L。结论 丁香酚、柠檬醛、肉桂醛和八角茴香油对口腔念珠菌均有抑菌作用，其中肉桂醛的抑菌作用最强，其次为柠檬醛和丁香酚，八角茴香油最弱。

关键词: 口腔念珠菌, 药物敏感性, 抗真菌中药

Abstract:

In vitro anti-candidal activity of the four traditional Chinese medicines. ZHENG Tong-jiao，ZHANG Hong. First Affiliated Hospital of DaLian Medical University，DaLian 116011，China
Abstract：Objective To estimate in vitro anti-candidal activity of Eugenol， Citral， Cinnamaldehyde and the Essential oil of Illicium verum， in order to pick the best one which has the highest sensibility to oral Candida spp. Also， to provide a foundation for further research and development of the anti-candidal traditional Chinese medicines. Methods With the broth microdilution testing procedure proposed by NCCLS， the minimum inhibitory concentration（MIC）of the four traditional Chinese medicines were determined in 38 clinically isolated Candida strains and 2 standard strains， and Fluconazole， with ATCC 6258 and ATCC 90028 used as positive control. Results For the Candida strains the MICs of Eugenol were 199.50 ~ 399.00 mg／L for C.albicans， 399.00 mg／L for C.krusei; the MICs of Citral were 166.31 ~ 332.63 mg／L for C.albicans， 166.31 mg／L for C.krusei; the MICs of Cinnamaldehyde were 23.55 ~ 94.2 mg／L for C.albicans， 47.10 mg／L for C.krusei; the MICs of the Essential oil of Illicium verum were 1218.75 ~ 2437.50 mg／L for C.albicans， 1218.75 mg／L for C.krusei. Conclusion Eugenol， Citral， Cinnamaldehyde and the Essential oil of Illicium verum have different anti-candidal effects on 38 strains Candida spp. The effect of Cinnamaldehyde is the best， followed by Eugenol and Citral， while the Essential oil of Illicium verum is the weakest.

Key words: oral Candida, antifungal susceptibility testing, antifungal traditional Chinese medicines