中国实用口腔科杂志

中国实用口腔科杂志

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实验性1型糖尿病大鼠牙槽骨高度变化研究

孙尚敏，闫露，潘亚萍，林莉，潘春玲，唐晓琳   

  1. 中国医科大学口腔医学院·附属口腔医院牙周病科，辽宁省口腔医学研究所牙周病研究室，辽宁  沈阳  110002
  • 出版日期:2017-03-15 发布日期:2017-04-11
  • 基金资助:

    辽宁省科学技术计划项目（2013225303）

  • Online:2017-03-15 Published:2017-04-11

摘要：

目的    探讨实验性1型糖尿病大鼠牙槽骨高度的变化。方法    选取SPF级雄性Sprague-Dawley（SD）大鼠12只，随机分为实验组和对照组，每组6只。实验组大鼠腹腔一次性注射新鲜配制的链脲佐菌素（STZ）（60 mg/kg）建立1型糖尿病模型；对照组大鼠注射相应体积的柠檬酸盐缓冲液。观察8周，然后处死所有大鼠并分离其上下颌骨，体式显微镜观察测量上下颌骨第一、二、三磨牙的牙槽骨高度，比较分析牙槽骨高度的变化。结果    对照组大鼠未见明显的牙槽骨破坏；实验组大鼠可见明显的牙槽骨破坏，破坏呈水平型，第一、二、三磨牙牙槽骨均有破坏，与对照组相比差异有统计学意义（P＜0.05）。结论    实验性1型糖尿病大鼠可见牙槽骨破坏，并且呈现缓慢的水平型吸收。

关键词: 糖尿病, 牙槽骨, 链脲佐菌素

Abstract:

Objective    To explore the alveolar bone height changes in experimental type 1 diabetic rats. Methods                 Twelve male Sprague-Dawley （SD） rats were randomly divided into experimental and control groups. The rats in the experimental groups were made type 1 diabetes mellitus（DM）by intraperitoneal injection of streptozotocin （STZ） （60mg/kg），while the control groups were injected with equal volume citrate buffer. All animals were sacrificed after 8 weeks. The alveolar bone loss（ABL） of the first molar，second molar and the third molar were measured from alveolar crest to enamel-cementum junction by stereoscope loupe. Results    The rats in the control groups had no obvious bone damage；while experimental groups showed obvious bone destruction horizontally， there was significant difference statistically（P＜0.05）. Conclusion    The alveolar bone damage was shown in rats with type 1 diabetes mellitus，and was horizontal absorption slowly .

Key words: diabetes mellitus, DM；alveolar；streptozotocin, STZ