关键词: 糖原合成酶激酶-3&beta, 炎性微环境, 成骨诱导

Abstract:

Objective    To discuss the influence of glycogen synthase kinase-3β（GSK-3β）on osteogenesis of jaw bone marrow mesenchymal stem cells（JBMMSCs） in inflammatory microenvironment. Methods    Use shRNA to knock down the expression of GSK-3β in JBMMSCs. IL-1β and TNF-α were used to mimic inflammatory microenvironment. After osteogenesis，Western Blot was used to detect osteogenesis-related protein expression and Alizarin Staining was used to detect the efficiency of osteogenesis in different groups. Results    Expression of osteogenesis-related protein and efficiency of osteogenesis was highly up-regulated after knocking-down of GSK-3β in JBMMSCs. Inflammatory microenvironment could significantly down-regulate the expression of osteogenesis-related protein and efficiency of osteogenesis in ordinary JBMMSCs，while knocking-down GSK-3β could attenuate this effect. Conclusion    Knocking-down of GSK-3β in JBMMSCs can both increase the efficiency of osteogenesis and resist the adverse effect of inflammatory microenvironment on osteogenesis，thus GSK-3β is a potential target for improving the regeneration in inflammatory microenvironment.

Key words: glycogen synthase kinase-3&beta, ；inflammatory microenvironment；osteogenesis