中国实用口腔科杂志

中国实用口腔科杂志

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人根尖牙乳头干细胞对T淋巴细胞增殖能力影响研究

刘雪梅，刘尧，姜力铭，丁振江，陈旭   

  1. 中国医科大学口腔医学院儿童口腔科，辽宁省口腔疾病重点实验室，辽宁省口腔疾病转化医学研究中心，辽宁  沈阳  110002
  • 出版日期:2017-01-15 发布日期:2017-03-28
  • 基金资助:

    国家自然科学基金青年科学基金（81600825）；辽宁省教育厅重点实验室基础研究项目（LS201610）

  • Online:2017-01-15 Published:2017-03-28

摘要：

目的    研究人根尖牙乳头干细胞（stem cells from apical papilla，SCAP）对小鼠脾脏来源的T淋巴细胞增殖能力的影响以及可能的机制，为揭示SCAP免疫调节性能的具体作用机制奠定基础。方法    原代分离培养人SCAP和小鼠脾脏来源T淋巴细胞。分为两个实验组，即SCAP与T淋巴细胞直接接触共培养组和transwell共培养组，以单独培养的T淋巴细胞作为对照组，流式细胞术检测T淋巴细胞增殖率。结果    在SCAP和T淋巴细胞共培养体系中，细胞直接接触共培养组T淋巴细胞的增殖率较对照组和transwell共培养组均明显降低，差异具有统计学意义（P < 0.05）；transwell共培养组T淋巴细胞的增殖率与对照组的差异无统计学意义（P > 0.05）。结论    人SCAP主要通过细胞间的直接接触作用抑制T淋巴细胞的增殖，进而发挥其免疫调节性能。

关键词: 根尖牙乳头干细胞, T淋巴细胞, 免疫调节

Abstract:

Objective    To investigate the effects of human stem cells from apical papilla（SCAP）on the proliferation rate of T lymphocytes derived from mouse spleen and the underlying mechanisms. Methods    Human SCAP and mouse T lymphocytes were isolated respectively and co-cultured either with cell-cell contact method or transwell method in vitro. Single cultured T lymphocytes were used as control group. The proliferation rate of T lymphocytes was evaluated using flow cytometery. Results    Compared with control group and transwell group respectively，the proliferation rate of T lymphocytes was significantly decreased in cell-cell contact group after co-cultured with SCAP（P < 0.05），while there was no significant change in transwell group（P > 0.05）. Conclusion    Human SCAP inhibits the proliferation of T lymphocytes mainly via cell-cell contact and further gets involved in immunomodulation.

Key words: stem cells from apical papilla, SCAP；T lymphocyte；immunomodulation