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02 January 2014, Volume 30 Issue 1 Previous Issue    Next Issue

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Time to rethink: transvaginal placement surgical mesh for pelvic floor reconstruction surgeries. ZHU Lan. 2014, 30(1): 12-14.  Abstract ( )   PDF (854KB) ( )   Abstract:FDA safety communication on serious complications associated with transvaginal placement of surgical mesh for pelvic organ prolapse accompany with some surgical mesh devices' delisting has caused enormous response from around the world, clinicians are confused with selections of reconstruction surgical procedures. Comprehensive understanding of background and Purpose of FDA Notification about transvaginal POP repair with mesh will help clinicians make right choice for pelvic floor reconstruction surgeries.

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Ovarian cancer stem cells and treatment of ovarian cancer.  YU Feng-sheng,LI Yi,CUI Heng. 2014, 30(1): 14-17.  Abstract ( )   PDF (871KB) ( )   Abstract:Cancer stem cells or tumor-initiating cells (CSC/TICs) play critical roles in tumorigenesis, traditional therapy resistance, tumor recurrence and metastasis. Major causes of poor survival rates of ovarian cancer patients are tumor recurrence and chemoresistance due to the existence of CSC/TICs. Treatment targeting both CSC/TICs and bulk tumor cells is thought to be more effective to eliminate ovarian tumors. Better understanding of ovarian CSC/TICs might facilitate the development of improved therapeutic strategies for ovarian cancer.

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The important issues on diagnosis and treatment of endometriosis.  LENG Jin-hua，DAI Yi. 2014, 30(1): 17-20.  Abstract ( )   PDF (870KB) ( )   Abstract:Endometriosis is a common disease in women of reproductive age, which induces chronic, inflammatory reaction and can impact on general physical, mental and social wellbeing. In this article, we reviewed the four important issues about management of pain, infertility, deeply infiltrating endometriosis and recurrent endometriosis based on updated researches reported in literature as well as our own experiences.

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Minimally invasive idea and minimally invasive surgery in gynecological treatment.  WANG Dan-bo,GUO Cui-shan. 2014, 30(1): 20-24.  Abstract ( )   PDF (879KB) ( )   Abstract:With the developing of medical technology, more and more minimally invasive surgeries are born, and patients have benefited from them. Widely performed minimally invasive surgeries including: intervention treatment, transvaginal surgery, laparoscopic surgery, minimally access surgery and etc. The minimally invasive idea is established because of the improvement of entire medical model based on minimally invasive techniques. With the minimally invasive idea, different but best minimally invasive surgeries are scientifically chosen to different diseases and “disease-centered” substitutes for “technique-centered”, enable patients to get the perfect therapeutic effect.

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The development of the diagnostic criteria of gestational diabetes mellitus and the challenges to clinical management.  ZHU Wei-wei,YANG Hui-xia. 2014, 30(1): 24-27.  Abstract ( )   PDF (872KB) ( )   Abstract:The diagnostic criteria of gestational diabetes mellitus has being changed continuously.Based on the hyperglycemia and adverse pregnancy outcome study,most international academic organizations including World Health Organization and Ministry in China published new criteria,while there are still some arguments.This article introduced the development of the diagnostic creiteria and discussed the challenges to our clinical management.

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Progress of study of placenta accreta.  FU Xiao-min,QI Hong-bo. 2014, 30(1): 27-30.  Abstract ( )   PDF (863KB) ( )   Abstract:Placenta accreta is a potentially life-threatening obstetric condition, the incidence of it has increased and seems to parallel the increasing cesarean delivery rate. It may be associated with massive and potentially life-threatening intrapartum and postpartum hemorrhage, disseminated intravascular coagulopathy, and puerperal infection that may require laparotomy and hysterectomy. It requires a multidisciplinary approach to management. A multidisciplinary team approach is essential to reduce neonatal and maternal morbidity and mortality.

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Renin-angiotensin system build on during pregnancy and origin and development of cardiovascular disease.  GAO Qin-qin,ZHANG Peng-jie,LI Ling-jun,XU Zhi-ce. 2014, 30(1): 31-34.  Abstract ( )   PDF (868KB) ( )   Abstract：Recently, more and more studies have demonstrated that a variety of intrauterine adverse environmental factors (including hypoxia, malnutrition and glucocorticoid) could alter renin-angiotensin system (RAS) and its functions during pregnancy. Most importantly, those RAS alterations may impact on cardiovascular functions in the offspring, and play a pivotal role in the pathophysiological development of various cardiovascular diseases in utero. This article provides a brief overview of the development of RAS during pregnancy and the importance of RAS in fetal origins of cardiovascular diseases.

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Progress of embryo implantation research.  QUAN Song,ZHANG Li-jia. 2014, 30(1): 34-38.  Abstract ( )   PDF (886KB) ( )   Abstract：Embryo implantation process includes positioning, adhesion and invasion. The regulatory mechanism of embryo implantation is complex and sophisticated, involving multiple genes, biological molecules, cytokines and signaling pathways. The embryo quality, endometrial receptivity, embryonic development, synchronization of endometrial development and good maternal-fetal crosstask are necessary for embryo implantation. Progress in basic and clinical research on embryo implantation help to further explore the mysteries of embryo implantation and gain a better understanding of the mechanism, which has important significance for diagnosis and treatment of infertility.

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Progress in postmenopausal hormone therapy.CHEN Rong.  CHEN Rong. 2014, 30(1): 38-40.  Abstract ( )   PDF (858KB) ( )   Abstract：Publication of the first results from the Women’s Health Initiative (WHI) trial in 2002 made menopausal management fall to peak negative sentiment. Over the past decade significant progress have been made in this area. The opinions about menopausal management are distinguished different from those around 2002. After discussion about design and limitations of WHI, the most important studies in the recent decade, e.g. reanalysis of WHI, Danish Osteoporosis Prevention Study and European active surveillance study of women taking HRT were detailed introduced. We emphasized that "opportunity window" theory and progestogens effects were the most important progress in the past decade. At the end of this article key points from international consensus about menopausal hormone therapy in 2013 were listed.

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The etiology of recurrent spontaneous abortion research.  XIAO Shi-jin,ZHAO Ai-min. 2014, 30(1): 41-45.  Abstract ( )   PDF (884KB) ( )   Abstract：Recurrent spontaneous abortion (RSA) is defined as the occurrence of three or more consecutive abortions with the same partner. Scholars in recent years have proposed that two consecutive spontaneous miscarriages should be included in the scope of RSA, because the abortion risk for future pregnancy is up to 50%,the clinical manifestation should be paid more attentionnow. RSA is a complex multifactorial problem associated with chromosome, reproductive tract anatomic, endocrinologic, infection, immune etiologies and pre-thrombotic state, but asubstantial proportion of RSA, up to 40%, are due to unexplained or unknown etiologies. The complex etiology made RSA one of diseases to be treated difficulty and a hot spots in the present clinical research work.

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Autophagy regulation in anoikis resistance for ovarian cancer.  YIN Xia,DI Wen. 2014, 30(1): 46-50.  Abstract ( )   PDF (865KB) ( )   Abstract：Objective To explore the role of autophagy in anoikis resistance for ovarian cancer cells as well its association with apoptosis.Methods Between June 2012 and June 2013 in Shanghai Jiaotong University School of Medicine,analysis of autophagy related protein and integrin related protein in A2780 ovarian cancer cells with different culture condition. siRNA and chemical inhibitor is used to investigate the regulation mechanism. real time PCR and western blot were used to investigate the expression of autophagy and integrin molecular in ovarian cancer cells, cells apoptosis is examined by flow cytometry.Results Comparing among routine culture, matrigel culture and suspend culture, the increasing LC3 expression of autophagy（[WTBX]P[WTBZ]< 0.05） accompanied with integrin CD51(αv)，CD61(β3) decreasing（[WTBX]P[WTBZ]< 0.05）, but there was no difference in protein expression of Beclin1 and CD29(β1) ([WTBX]P[WTBZ]>0.05). Inhibiting integrin αvβ3 pathway by RGD，autophagy protein expression of LC3 was increased（[WTBX]P[WTBZ]<0.05）. RGD promoted cell apoptosis with autophagy inhibitor 3-MA（[WTBX]P[WTBZ]<0.05）,but not without 3-MA([WTBX]P[WTBZ]>0.05).Conclusion Autophagy pathway played a potentially protective role in anoikis apoptosis.Intercellular signaling pathways of integrin might play a regulatory role on autophagy in ovarian cancer anoikis resistance which resulted in distant metastasis of cancers.

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Study of target gene screening of miR-134 in ovarian cancer chemoresistance.  SHUANG Ting, WANG Min,CHANG Shuang, ZHOU Ying-ying, YAN Xiao-yu, SHI Cong, HU Ting. 2014, 30(1): 51-54.  Abstract ( )   PDF (861KB) ( )   Abstract：Objective Applying Hybrid-PCR method to screen target genes of miR-134 in ovarian cancer chemoresistant cells and to provide a theoretical basis on the formation of ovarian cancer chemoresistance.Methods RNA extraction, primer design, Hybrid- PCR was carried out to screen putative targets of miR-134 in SKOV3-TR30 cells,Dual Luciferase Reporter Assay System was used to evaluate the binding of miR-134 with 3’UTR of candicate targets predicated by Hybrid-PCR.Results By applying Hybrid -PCR we find in SKOV3-TR30 cells target mRNA of miR-134：C16orf72, PNAS-105,spermidine synthase, VIM2,F-box protein 2,GAPDH,PRPF6 and RPL41. We successfully constructed 3'-UTR plasmids of the 8 putative targets of miR-134 in SKOV3-TR30 cells.By co-transfecting mRNA 3'UTR PMIR-REPORT and Psilencer-miR-134 to HEK293 cell respectively, we demonstrated that miR-134 directly binds to 3'UTR of these 5 mRNAs.Conclusion Hybrid-PCR is an effective and rapid approach for screening putative miRNA targets and could be applied to find target mRNA of miRNA in SKOV3-TR30 cells. C16orf72, PNAS-105,spermidine synthase, VIM2,F-box protein 2,GAPDH,PRPF6 and RPL41 could be target mRNA of miR-134 in SKOV3-TR30 cell and miR-134 could take part in chemoresistance formation of SKOV3-TR30 cells via these targets.