中国实用妇科与产科杂志

• 论著 • 上一篇    下一篇

microRNA-145对卵巢癌SKOV3细胞抑癌基因p53表达调控研究

刘龙浩a魏莎莉a余秋波b陈渝a刘祖翠a高唐鑫子a   

  1. 作者单位:重庆医科大学, a 干细胞与组织工程研究室, b 生命科学研究院,重庆 400016
  • 出版日期:2015-01-02 发布日期:2015-01-05
  • 基金资助:

    国家自然科学基金(81100443);重庆市渝中区科技计划项目(20120214)

Regulation of miR-145 on p53 in SKOV3 cell of ovarian cancer.

LIU Long-hao,WEI Sha-li,YU Qiu-bo,CHEN Yu,LIU Zu-cui,GAOTANG Xin-zi.   

  1. Chongqing Medical University, Chongqing 400016 ,China
  • Online:2015-01-02 Published:2015-01-05

摘要:

目的 探讨microRNA -145(miR-145)对卵巢癌SKOV3细胞抑癌基因p53表达的调控。 方法 选取2012年2月至2013年4月在重庆医科大学,通过慢病毒转染技术将含miR-145的质粒转入SKOV3细胞中,经嘌呤霉素筛选建立稳定表达miR-145的SKOV3细胞株。实时定量荧光RT-PCR验证miR-145的表达情况,western-blot检测过表达miR-145后SKOV3细胞中p53蛋白表达量的变化。 结果 过表达miR-145的SKOV3细胞p53蛋白相对表达量实验组0.714±0.18,对照组0.333±0.07,增高1.14倍(P<0.05)。 结论 miR-145可能通过增强卵巢癌SKOV3细胞中抑癌基因p53的表达来降低细胞的肿瘤恶性程度。

关键词: microRNA -145, 卵巢肿瘤, SKOV3, p53

Abstract:

Abstract: Objective To explore regulation of miR-145 on p53 in SKOV3 cell of ovarian cancer. Methods With lentivirus transfection technology plasmids of miR-145 were transformed into SKOV3 cell,and by puromycin establish SKOV3 cell strains to express miR-145 steadily. Prove the expressing situation of miR-145 by RT-PCR, relative expression of p53 of SKOV3 after the overexpression of miR-145 was analysed by western-blot from February 2012 to April 2013.Results The expression of protein p53 of SKOV3 increased 1.14 times compared with control group after the overexpression of miR-145 (P<0.05).Conclusion The miR-145 may weaken the malignancy degree by increasing the expression of suppressor gene p53 in SKOV3 cell of ovarian cancer.

Key words: micro RNA-145;ovarian cancer, SKOV3;p53

中图分类号: