红核妇洁洗液对阴道加德纳菌及其生物膜作用的研究

宗晓楠; 赵晓雯; 尚翔; 白会会; 范琳媛; 刘朝晖

doi:10.19538/j.fk2026010121

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中国实用妇科与产科杂志 ›› 2026, Vol. 42 ›› Issue (1) : 100-104. DOI: 10.19538/j.fk2026010121

论著

红核妇洁洗液对阴道加德纳菌及其生物膜作用的研究

宗晓楠 ^1a ,
赵晓雯 ^1a ,
尚翔 ² ,
白会会 ^1b ,
范琳媛 ^1a ,
刘朝晖 ^1a

作者信息 +

Study on the effect of Honghe Fujie Lotion on gardnerella vaginalis and its biofilm

Author information +

文章历史 +

摘要

目的探讨红核妇洁洗液（以下简称红核）对阴道加德纳菌及其生物膜的作用。方法选取2022年11月至2023年6月间在首都医科大学附属北京妇产医院初次诊断为细菌性阴道病（BV）患者576例，从其阴道分泌物中分离纯化并鉴定出加德纳菌10株，将红核与阴道加德纳菌共培养48h或者红核作用于已培养24h的阴道加德纳菌后，检测光密度（OD）值、抑菌率、最小抑菌浓度（MIC）、最小抑制生物膜浓度（MBIC），并通过扫描电镜观察阴道加德纳菌的生物膜形成情况。结果阴道加德纳菌生物膜形成能力在培养48h时最强（P<0.05）。治疗剂量（原液稀释10倍）红核对阴道加德纳菌0~5 min杀菌率均高达94%以上，具有快速、强效、稳定的杀菌作用。红核在低浓度下即可有效抑制阴道加德纳菌生长及生物膜形成，对不同菌株的抑菌率有所差异，MIC波动在1/1280~1/40之间，MBIC值为稀释1/80，均明显低于治疗剂量。在扫描电镜下观察发现，浓度低于治疗剂量的红核仍能对已形成的阴道加德纳菌生物膜产生一定的破坏作用。结论红核对阴道加德纳菌有很好的杀菌作用，并且能够有效抑制生物膜形成及对于已形成的成熟生物膜具有破坏作用。

Abstract

Objective To investigate the effects of Honghe Fujie Lotion on Gardnerella vaginalis and its biofilm. Methods A total of 576 patients initially diagnosed with bacterial vaginosis at the Beijing Obstetrics and Gynecology Hospital，Capital Medical University，between November 2022 and June 2023 were enrolled. Ten strains of Gardnerella vaginalis were isolated，purified，and identified from their vaginal secretion samples. Honghe Fujie Lotion was co-cultured with Gardnerella vaginalis for 48 hours or applied to Gardnerella vaginalis pre-cultured for 24 hours. Parameters including OD value，inhibition rate，minimum inhibitory concentration （MIC），and minimum biofilm inhibitory concentration （MBIC） of Gardnerella vaginalis were measured. The status of vaginalis biofilm was observed by scanning electron microscopy. Results The biofilm formation capacity of Gardnerella vaginalis was the strongest after 48 hours of culture （P<0.05）. At the therapeutic dose （10%），the bactericidal rate exceeded 94% within 0-5 minutes，，which showed a rapid，strong and stable bactericidal effect of Honghe Fujie Lotion. Honghe Fujie Lotion could effectively inhibit the growth and biofilm formation of Gardnerella vaginalis even at a low concentration. The inhibitory rates for different strains were different. The MIC value of it ranged from 1/40 to 1/1280 and MBIC value was at 1/80 dilution，still significantly lower than the therapeutic dose. Under the scanning electron microscope，it was found that, even at a concentration lower than the therapeutic dose, Honghe Fujie Lotion could still exert a significant disruptive effect on Gardnerella vaginalis biofilm. Conclusion Honghe Fujie Lotion has a good bactericidal effect on Gardnerella vaginalis and can effectively inhibit the formation of biofilm and destroy the mature biofilm.

导出引用

宗晓楠, 赵晓雯, 尚翔, 等. 红核妇洁洗液对阴道加德纳菌及其生物膜作用的研究[J]. 中国实用妇科与产科杂志. 2026, 42(1): 100-104 https://doi.org/10.19538/j.fk2026010121

ZONG Xiao-nan, ZHAO Xiao-wen, SHANG Xiang, et al. Study on the effect of Honghe Fujie Lotion on gardnerella vaginalis and its biofilm[J]. Chinese Journal of Practical Gynecology and Obstetrics. 2026, 42(1): 100-104 https://doi.org/10.19538/j.fk2026010121

中图分类号： R711.31

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