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06 September 2006, Volume 21 Issue 09 Previous Issue    Next Issue
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Analysis of related factors with 2D ultrasonography diagnosis in the craniocerebral injury after newborn asphyxia. Li Huiming,Xi Binrong,Liu Huiru 2006, 21(09): 656-658 .  Abstract ( )   AbstractObjectiveTo investigate the correlation of craniocerebral injury to asphyxia in newborn. MethodsA total of 196 cases of newborn head were inspected with 2D ultrasonography for craniocerebral injury;with combination of clinical materials,the comparison of craniocerebral injury in asphyxia group was contrasted with the control group. ResultsThere were 73 cases of craniocerebral injury in 196 cases of newborn.Among them,there were 54 cases of craniocerebral injury in all the 90 cases (60%) of premature newborn,and 19 in all the 106 (18.49%) of mature newborn.The craniocerebral injury of newborn asphyxia was significantly positively correlated with fetal age to fetal weight (P<0.01).The incidence of craniocerebral injury in premature newborn or in low weight newborn was significantly higher than that in mature newborn or in normal weight newborn (P<0.05,or P<0.01).The incidence of craniocerebral injury in premature newborn was not significantly different from that in low weight newborn between control group and asphyxia group (P>0.05); and the incidences of craniocerebral injury in mature newborn,normal weight newborn and abdominouterotomy newborn were significantly different between control group and asphyxia group (each P<0.05). ConclusionThe newborn asphyxia is one of the major factors of craniocerebral injury in mature newborn,normal weight newborn and abdominouterotomy newborn; to decline the births of premature newborn and of low weight newborn is to make the incidence of craniocerebral injury decreased efficiently; the head ultrasonography is one of the important methods which may be used to make the craniocerebral injury diagnosed,screened and pursued.

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Analysis of related factors with 2D ultrasonography diagnosis in the craniocerebral injury after newborn asphyxia. Li Huiming,Xi Binrong,Liu Huiru 2006, 21(09): 656-658 .  Abstract ( )   AbstractObjectiveTo investigate the correlation of craniocerebral injury to asphyxia in newborn. MethodsA total of 196 cases of newborn head were inspected with 2D ultrasonography for craniocerebral injury;with combination of clinical materials,the comparison of craniocerebral injury in asphyxia group was contrasted with the control group. ResultsThere were 73 cases of craniocerebral injury in 196 cases of newborn.Among them,there were 54 cases of craniocerebral injury in all the 90 cases (60%) of premature newborn,and 19 in all the 106 (18.49%) of mature newborn.The craniocerebral injury of newborn asphyxia was significantly positively correlated with fetal age to fetal weight (P<0.01).The incidence of craniocerebral injury in premature newborn or in low weight newborn was significantly higher than that in mature newborn or in normal weight newborn (P<0.05,or P<0.01).The incidence of craniocerebral injury in premature newborn was not significantly different from that in low weight newborn between control group and asphyxia group (P>0.05); and the incidences of craniocerebral injury in mature newborn,normal weight newborn and abdominouterotomy newborn were significantly different between control group and asphyxia group (each P<0.05). ConclusionThe newborn asphyxia is one of the major factors of craniocerebral injury in mature newborn,normal weight newborn and abdominouterotomy newborn; to decline the births of premature newborn and of low weight newborn is to make the incidence of craniocerebral injury decreased efficiently; the head ultrasonography is one of the important methods which may be used to make the craniocerebral injury diagnosed,screened and pursued.

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Exploration of the diagnosis of 640 cases of cerebral palsy. Xu Zhizhi,Zhou Xiaoyu,Jiang Xiaoyong 2006, 21(09): 659-662 .  Abstract ( )   AbstractObjectiveTo explore the early diagnosis of cerebral palsy. MethodsA total of 640 cases of diagnosed cerebral palsy were divided 2 groups.They were 258 premature infants(PI) group and 382 mature infants(MI) group.Physical neurological examination,intelligence test(CDCC) and cranial CT examination were given to each case. Results1.High risk factors group(PI) was higher than MI group in intracranial hemorrhage and pulmonary disease but MI group was higher than group PI in hypoxicischemic encephalophathy (P all were <0.05),others P all were>0.05.2.The clinical features of cerebral palsy of those diagnosed within 6 monthes in groups PI and MI were shown in 28 and 70 cases respectively.Group PI was more severe than group MI in poor feeding、crying、moving、less reaction and hypotonic but group MI was more severe than group PI in being noisy whole day、nursy difficulty and hypertonic,P all were <0.05 and <0.01,others P all were >0.05.3.Physical and intelligence developments:Group PI and MI in body length(cm)、body weight(kg)、head circumference(cm),MDI and PDI(mark) were 73.24±9.82、73.58±11.64、9.12±2.31、9.23±2.80、44.15±3.47、42.99±4.23、44.42±6.09、41.99±7.28、43.81±4.95、40.80±4.51 respectively.4. The abnormality features of cranial CT scan was 95% in group PI and 89.8% in group MI P<0.05.5.Retardation:Group PI was more SEVERE than group MI in language retardation,group MI was more severe than group PI in body length(<2SD),P all were<0.05. ConclusionThe cerebral palsy can be early diagnosed by infant with high risk factors,abnormal physical intelligence development,neurological manifestation and abnormal cranial CT.Once diagnosis is done,early intervention should be given.

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Changes of extracellular matrix components in bronchoalveolar lavage fluid in premature infants with chronic lung disease. Zhang Qianshen,Chang Liwen,Rong Zhihui. 2006, 21(09): 663-665 .  Abstract ( )   AbstractObjectiveTo clarify the roles of HA、PCⅢ、Hyp in bronchoalveolar lavage fluid with chronic lung disease(CLD) of prematurity and their effect on the progression of CLD. MethodsLung lavage was serially obtained every day from 62 ventilated premature infants that were hospitalized in NICU from December 1999 to November 2004. Total cell counts, neutrophilic granulocyte counts, HA、PCⅢ、Hyp in bronchoalveolar lavage fluid were examined. Among the 62 cases, 20 developed pneumonia, 13 HMD, 15 CLD and 14 without lung disease as control. ResultsThe levels of HA、PCⅢ、Hyp in CLD group were not only significantly higher than those in HMD、pneumonia group, but also significantly higher than those in BALF in control group. In CLD group, the levels of HA and Hyp in BALF were respectively correlated with total cell numbers and neutrophils. PCⅢlevels were related to alveolar macrophages. Also, there were positive correlation between PCⅢ、HA and Hyp in BALF. ConclusionThese results suggest that the production of PCⅢ、HA and Hyp in lung in CLD were increased and that the levels of PCⅢ、HA、Hyp in BALF might reflect the activity of the disease from different aspects and play a role in estimating prognosis.

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Observe and analyse the clinical curative effect of using GM1 on treating premature white matter damage. Jin Yi,Wang Ying,Zhang Xin 2006, 21(09): 666-668 .  Abstract ( )   AbstractObjectiveTo study on the clinical significance of GM1 in treating the premature white matter damage. MethodsAll premature infants who were hospitalized in NICU received routine bedside cranial sonography detection before 1 weekaged.Divide the confirmed patients into GM1 treating group and control group.The treating group consisted of 56 cases;control group of 33 cases.On the basis of being positive treated complicating disease,the treating group also accepted the treatment of GM1.Dynamically observe the changing of cranial sonography and follow up. ResultsOf 356 cases,89 were confirmed,and the proportion was 25%.After one course of treatment observe the recovery of cranial sonography.The treating group was better than the control group.The divergence had prominence.Thirtythree cases of treating group were followed up to 6 monthaged.Sixteen cases had ventriculus enlarging,covering 48%;2 cases of obvious locomotion and intelligence hypoevolutism;5 cases of localized abnormality of muscular tension 、 locomotion and slight disturbance of cognition.In control group,10 cases were followed to 6 monthaged.Eight cases occurred ventriculus enlarging,covering 80%.Three cases appeared obvious locomotion and intelligence hypoevolutism;2 cases occurred slight disturbance of cognition and behavior. ConclusionGM1 can decrease the occurrence of periventricular leukomalalia and continued white matter damage.

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Result of cranial Bultrasonography and clinical analysis of 404 cases of preterm infants. Wang Le,Li Mingxia,Zhou Ying. 2006, 21(09): 669-672 .  Abstract ( )   AbstractObjectiveTo investigate the incidence of brain injury and risk factors. MethodsAll 404 cases hospitalized preterm infants were conventionally examined by the beside cranial ultrasound between 3 to 7 days after birth from Aug.2003 to Oct.2005. ResultsBrain injury was diagnosed in 150 cases.The mean gestational age of them was (33.27±1.99)weeks,and birthweight (1992.9±505.2)g.Total incidence of IVH and PVL was 35.2%(142/404) and 3.5%(14/404) respectively among all preterm infants,and the proportion of slight and severe brain injury was 23.5%(95/404) and 13.6%(55/404) respectively.The lower the gestational age and birthweight,the higher the incidence of brain injury,but it was not correlated with the level of IVH.The degree of asphyxia was closely related to the level of brain injury.The incidence of complications,such as RDS,apnea,respiratory failure,pulmonary hemorrhage,hypoglycemia,multiorgan function failure and anemia was related to the degree of brain injury.Pregnancyinduced hypertension syndrome,cholestasis,premature rupture of membranes,gestational age,birthweight and highfrequency oscillatory ventilation(HFOV) could make the incidence of brain injury higher. ConclusionThe incidence and level of preterm brain injury are related to many factors.We could diagnose early by cranial ultrasound and make it possible for us to intervene and treat these patients as early as possible in order to prevent the bad prognosis.

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Study on oxacillinresistance of staphylococcus aureus and detection of mecA gene. Yang Rongwang,Hua Chunzhen,Mao Shujiong 2006, 21(09): 673-676 .  Abstract ( )   AbstractObjectiveTo study the antimicrobial agents resistance of staphylococcus aureus in Hangzhou area and the sensitivity and specificity of the mecA gene PCR assay. MethodsA total of 133 staphylococci strains were isolated from the children in Hangzhou,then Disc Diffusion and staphylococcal mecA gene PCR assay for detecting MRSA were compared with Etest method. ResultsOf all 133 strains performed with both KirbyBauer and Etest methods in the susceptibility test,85.0% and 86.5% of the isolates were susceptible to oxacillin respectively.All of the isolates were susceptible to vancomycin with Etest MICs ≤4.0 ug/ml,and 91.7% of them had the zone diameters not more than 15mm.The sensitivity of Disc Diffusion and mecA gene PCR assay for detecting MRSA were 100% respectively,the Disc Diffusion specificity was 96.6%,and the mecA gene PCR assay's specificity was up to 98.3%. ConclusionRate of oxacillin resistance staphylococcus aureus is low in the study,and mecA gene PCR assay is significant in rapid detection of MRSA.

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Analysis of 35 neonates with infection caused by staphylococcus aureus and investigation on antibioticresistance and mecA gene of those strains. Hua Chunzhen,Yu Huimin. 2006, 21(09): 677-679 .  Abstract ( )   AbstractObjectiveTo analyze the clinical manifestation of newborns with infection caused by staphylococcus aureus (S. aureus) and investigate the antibioticresistance phenotype of these strains. MethodsS. aureus isolates were identified with GPI card of Vitek system. Antibiotics sensitivity test were performed by using disk diffusion methods,and sensitivity test of oxacillin and vancomycin was performed with a further Etest method.The mecA gene was detected with PCR. ResultsThirtyfive neonates (21 males and 14 females) with infection caused by staphylococcus aureus were identified, aged from 6d to 28d,including 24 cases of pneumonia, 7 cases of septicaemia, 2 cases of hypodermic gangrene, 1 case of phlegmona with formation of an abscess and 1 case of omphalitis. Drug susceptibility test showed that 97.1% of 35 strains were βlactamasepositive and resistant to penicillin, while 82.9% of all strains were sensitive to oxacillin with MIC50 0.5 μg/m and MIC90 4.0 μg/mL. All strains were sensitive to vacomycin and rifampin , while 82.9% of all strains were susceptible to cefotaxime and ceftriaxone. Resistance rates to erythromycin, tetracycline, clindamycin, trimethoprimsulfamethoxazole, ofloxacin and chloramphenicol were 54.3%, 37.1%, 17.1%, 14.3%, 5.7% and 2.9% respectively.The mecAPCR showed that the control strain ATCC25923 and all oxacillinsensitive S. aureus were mecAnegative ,while 6 oxacillinresistant strains were mecApositive. ConclusionLow respiratory tract infection is the most common disease in all infections caused by S. aureus in newborn children and most of them are community acquired.

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Analysis of 35 neonates with infection caused by staphylococcus aureus and investigation on antibioticresistance and mecA gene of those strains. Hua Chunzhen,Yu Huimin. 2006, 21(09): 677-679 .  Abstract ( )   AbstractObjectiveTo analyze the clinical manifestation of newborns with infection caused by staphylococcus aureus (S. aureus) and investigate the antibioticresistance phenotype of these strains. MethodsS. aureus isolates were identified with GPI card of Vitek system. Antibiotics sensitivity test were performed by using disk diffusion methods,and sensitivity test of oxacillin and vancomycin was performed with a further Etest method.The mecA gene was detected with PCR. ResultsThirtyfive neonates (21 males and 14 females) with infection caused by staphylococcus aureus were identified, aged from 6d to 28d,including 24 cases of pneumonia, 7 cases of septicaemia, 2 cases of hypodermic gangrene, 1 case of phlegmona with formation of an abscess and 1 case of omphalitis. Drug susceptibility test showed that 97.1% of 35 strains were βlactamasepositive and resistant to penicillin, while 82.9% of all strains were sensitive to oxacillin with MIC50 0.5 μg/m and MIC90 4.0 μg/mL. All strains were sensitive to vacomycin and rifampin , while 82.9% of all strains were susceptible to cefotaxime and ceftriaxone. Resistance rates to erythromycin, tetracycline, clindamycin, trimethoprimsulfamethoxazole, ofloxacin and chloramphenicol were 54.3%, 37.1%, 17.1%, 14.3%, 5.7% and 2.9% respectively.The mecAPCR showed that the control strain ATCC25923 and all oxacillinsensitive S. aureus were mecAnegative ,while 6 oxacillinresistant strains were mecApositive. ConclusionLow respiratory tract infection is the most common disease in all infections caused by S. aureus in newborn children and most of them are community acquired.

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Analysis of 35 neonates with infection caused by staphylococcus aureus and investigation on antibioticresistance and mecA gene of those strains. Hua Chunzhen,Yu Huimin. 2006, 21(09): 677-679 .  Abstract ( )   AbstractObjectiveTo analyze the clinical manifestation of newborns with infection caused by staphylococcus aureus (S. aureus) and investigate the antibioticresistance phenotype of these strains. MethodsS. aureus isolates were identified with GPI card of Vitek system. Antibiotics sensitivity test were performed by using disk diffusion methods,and sensitivity test of oxacillin and vancomycin was performed with a further Etest method.The mecA gene was detected with PCR. ResultsThirtyfive neonates (21 males and 14 females) with infection caused by staphylococcus aureus were identified, aged from 6d to 28d,including 24 cases of pneumonia, 7 cases of septicaemia, 2 cases of hypodermic gangrene, 1 case of phlegmona with formation of an abscess and 1 case of omphalitis. Drug susceptibility test showed that 97.1% of 35 strains were βlactamasepositive and resistant to penicillin, while 82.9% of all strains were sensitive to oxacillin with MIC50 0.5 μg/m and MIC90 4.0 μg/mL. All strains were sensitive to vacomycin and rifampin , while 82.9% of all strains were susceptible to cefotaxime and ceftriaxone. Resistance rates to erythromycin, tetracycline, clindamycin, trimethoprimsulfamethoxazole, ofloxacin and chloramphenicol were 54.3%, 37.1%, 17.1%, 14.3%, 5.7% and 2.9% respectively.The mecAPCR showed that the control strain ATCC25923 and all oxacillinsensitive S. aureus were mecAnegative ,while 6 oxacillinresistant strains were mecApositive. ConclusionLow respiratory tract infection is the most common disease in all infections caused by S. aureus in newborn children and most of them are community acquired.

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Comparing the ASSURETM RAPID test with other diagnostic methods for helicobacter pyloric infection in children Su Lin,Liu Wei,Zhang Yin 2006, 21(09): 680-682 .  Abstract ( )   AbstractObjectiveIn this study,we compared the ASSURETM RAPID test,a serological assay for antiH·pyloric antibodies,with severl methods which are commonly used in pediatrics. MethodsOur study has recuited 100 patients (age 3~16 years old, 47 male, and 53 female) with upper gastrointestinal symptoms. We tested them for H·pyloric with rapid urease test(CLO test),histological examination of gastric biopsy sample, and anti-H.pyloric antibodies. ResultsThe sensitivities,CLO test, histological examination, 13Curease breath test and ASSURETM RAPID test,were81.48%,75.39%,96.3%,94.50%.specificities,were86.96%,80.43%100%,91.11%, respectively. ConclusionFurthermore, ASSURETM RAPID test, was equally effective with either whole blood or serum samples. With the convenience in its administration and high degree of sensitivity and specificity, we concluded that ASSURETM RAPID test is highly desirable for studying the epidemiology of H.pyloric infections where medical facilities and finacial resources are limited.

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Detecting the variation of intestinal microflora in children with rotavirus enteritis by 16SrRNA fluorescent quantificative PCR. Yang Meifen,Huang Yongkun，Wang Yuming 2006, 21(09): 683-686 .  Abstract ( )   AbstractObjectiveTo study the variation of intestinal microflora in children with rotavirus enteritis and to understand practicality and feasibility of fluorescent quantificative PCR in the field. MethodsThe bifidobacteria，lactobacillus and escherichia coli in children with rotavirus enteritis and healthy children were detected by 16SrRNAtargeted PCR，and were compared with the result by the conventional method. ResultsCompared with the healthy children，the number of bifidobacteria and lactobacillus appeared significant variation in patients(P<0.05).However,the number of escherichia coli had no significant variation(P>0.05),and the range of bacteria by fluorescent quantificative PCR was nearly equal to the result by the cultivation techniques from other document. ConclusionThe number of probiotics in patients is less than the control.Fluorescent quantificative PCR is a method with high specificity and sensitivity，and it can be applied to the quantification of bacteria in the intestine and saves more time and labor.

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Effect of meropenem on lower respiratory infection in newborn infants with mechanical ventilation. Shi Yuan,Zhao Jinning,Tang Shifang 2006, 21(09): 687-688 .  Abstract ( )   AbstractObjectiveTo explore the effect of meropenem on lower respiratory infection in newborn infants with mechanical ventilation. MethodsThirtyseven mechanical ventilated newborn infants with lower respiratory infection were involved in this study.When the patients were treated with mechanical ventilation,they were administrated by venous injection of meropenem with a dose of 20~30mg/kg,twice a day.The evaluation of clinical effect was divided into four degrees: healing,remarkable effect,advancement,and invalidation.The degree of healing and remarkable effect was appraised as effective. ResultsAt the end of the course with meropenem,19 newborns were healed,7 had remarkable effect,2 had advanced effect,and 9 had no effect.The total effect rate of meropenem reached 70.3%.No side effect was observed. ConclusionMeropenem is suitable for treating mechanical ventilated newborn infants with lower respiratory infection,and worth further investigating.

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Gastric acid secretion from neonate to childhood Chen Shuzhen 1,Jiang Mizu 2. 2006, 21(09): 691-692 .  Abstract ( )   AbstractObjectiveTo investigate the gastric acid secretion from neonate to childhood. MethodsIntragastric pH was recorded by ambulatory pH monitoring (Medtronic) in 982 children. ResultsIntragastic pH values were 2.32±0.90、1.92±0.58、1.66±0.63、1.70±0.48、1.59±0.47 in infants for neonates and different groups aged less than 1 year old,3 years old,7 years old,15 years old,respectively.The difference between the neonates and other groups was significant (P<0.001),and was also observed between the group aged less than 1 year old and other groups whereas the difference among groups aged less than 3 years old,7 years old 15 years old had no any significant.The values of gastric pH in neonates less than two weeks and larger than two weeks were 2.61±0.93、2.38±0.92,respectively.No significant difference was observed between these two groups. ConclusionGastric acid secretion were observed in the newborn from the second day of life.The values of gastric pH is significantly higher than that in both infant and older children.Gastric pH values decreases with the development of children,and by 1 year old gastric acid secretion approaches that in adults.Our results suggests that acidrelated diseases may occur in all the infants including neonates.

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The research of Helicobacter pylori vacA genotypes in children. Tang Qing,Wang Linling,Liang Danmei 2006, 21(09): 693-695 .  Abstract ( )   AbstractObjectiveTo investigate the distribution of H·pylori vacA genetypes and their association with gastric duodenal diseases in children. MethodsEighty biopsy samples from patients with H·pylori infection were studied.PCR analysis for vacA genotypes was performed by using DNA extracted from biopsy specimens. ResultsIn 80 cases,there were two vacA mosaicisms including 3 for s1a/m1(3.75%) and 55 for s1a/m2(66.25%).No statistical differences were found in the distribution of vacA genotype of H·pylori strain infection between chronic superficial gastritis and peptic ulcer(P>0.05). ConclusionIn Nanning,vacAs1a/m2 genotype of H·pylori is predominant in children.Part of patients may be coinfected with different vacA genotypes of H·pylori.The vacA genotype can not be considered the markers of H·pylori which is more pathogenic in children in Nanning.

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Infectious mononucleosis and related diseases. Wang Xiaoli,Yu Jie，Xu Youhua 2006, 21(09): 696-695 .  Abstract ( )   AbstractObjectiveInfectious mononucleosis is becoming more common in pediatric clinic.The study analyzed the manifestation of EBV related diseases to provide references for the clinical management. MethodsAll 602 cases were grouped with IM (n=279),IMlike disease (n=80 ) and EBV infection (n=243),and were evaluated retrospectively.SPSS11.5 software was used for statistical analysis. ResultsThe percent of typical clinical findings in IM group was significantly higher than those in EBV infection group,but only the percent of enlarged lymph nodes and eyelid edema was higher than those in IMlike group.The percent of HPVB19 in IMlike group (54.8%) was significantly higher than that in IM group.The liver function with abnormal hepatic enzymes was the most common results in the three groups and LDH abnormality was the most significant.The percent of anemia,granulopenia and thrombocytopenia in the three groups showed no significant differences.The percent of pneumonia in IMlike group was significantly higher than that in IM group. ConclusionEyelid edema shows the same important value as the other typical manifection for IM diagnosis.HPVB19 is the most common pathogens in IMlike disease.The mechanism for the patients with EBV infection and IM disease may attribute to the different immune state.

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Clinical value of videoEEG for the children with episodic events. Zhuang Jiaxin,Lin Caimei,Wang Kunming 2006, 21(09): 699-700 .  Abstract ( )   AbstractObjectiveTo evaluate the clinical value of videoEEG for the children with episodic events. MethodsFrom May 2005 to Dec.2005,videoEEG monitoring was done in 163 children with episodic events in the Quanzhou Children’s Hospital and a retrospective analysis was carried out. ResultsAmong 163 cases,40 seizures (24.5%) were recorded,23 of whom (14.1%) were confirmed as epileptic seizures,and 17 of whom (10.4%) were confirmed as nonepileptic seizures.Totally 123 patients (75.4%) were confirmed as epilepsy,35 of whom (35/123,28.4%) were clarified as epilepsy syndrome. ConclusionVideoEEG is a direct method of differential diagnosis for the children with episodic events,and is a reliable way for the typing of childhood epilepsy and the classification of epileptic syndrome.

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Internet addiction disorder behavioral trait and the family education nethods in adolescents. Yan Chunmei,Yang Fengchi. 2006, 21(09): 701-703 .  Abstract ( )   AbstractObjectiveto investigate the Internet Addiction Disorder (IAD) behavioral trait and the family education methods,in order to find out the susceptible factors of IAD. MethodsThe 46 patients in the adolescence clinic were diagnosed and analyzed by means of the diagnosis criteria about the IAD,and some investigations were conducted by filling the Child Behavior Checklist (CBCL) and the parent bring-up ways evaluation forms (EMBU),comparing with the normal group. ResultsThe social condition in the social ability was dramatically different from those in control group (P<0.05).The flinch,the signs complain,the anxiety and depress,the attention problem and attack action in the behavior ability were dramatically different from those in control group (P<0.05).There was significant difference between IAD and normal groups by comparison of parent education methods.The fatherincharge group showed finicky interpose and overprotection,and the mother-in-charge group showed sensibility warmth has reduced,the excess intervention,and denial and refusal.The IAD society adaptability and behavior capability were obviously different from the normal groups (P<0.05). ConclusionThe IAD teenagers may have some defecs in the society adaptability and behavior capability.The IAD of teenagers are closely related to the family education methods,which affect the forming of the children's personality.