Abstract: Objective　To investigate the relationship of CD25 expression in peripheral blood T cells and serum CD25 level with nephritis with Henoch-Schonlein purpura（HSP）in children，in order to help evaluate HSP. Methods　Forty children diagnosed with HSP were collected as study group and divided into two groups according to renal involvement：HSPN group（18 cases） and NHSPN group（22 cases）. During the same period，25 healthy children were taken as control group. The expression of CD25 in peripheral blood T cells was detected by flow cytometry. Serum CD25 levels were determined by enzyme-linked adsorption（ELISA）. The differences and relationship between the two groups were compared. Results　Compared with the control group，the expression of CD25 in peripheral blood of HSP children were decreased in CD3+ total T cells［HD（2.19±0.85）%、HSP（1.43±0.78）%］，CD3+CD4+T cells［HD（3.82±2.18）% 、HSP（2.40±0.95）%］ and CD3+CD4-T cells［HD（1.90±0.51）%、HSP（1.25±0.35）%］（P<0.05）. The expression of CD25 in peripheral blood CD3+CD4+T cells［NHSPN（2.62±1.03）% 、HSPN（2.11±0.78）%］ and the level of serum CD25［NHSPN（649.0±346.5）pg/mL、HSPN（407.0±141.0）pg/mL］ in HSPN children were lower than those in NHSPN children（P<0.05）. Serum CD25 level was negatively correlated with IgA level in HSP children（r=-0.5060，P<0.05），but was the expression of CD25［NHSPN（649.0±346.5）pg/mL、HSPN（407.0±141.0）pg/mL］ in peripheral blood T cells was not related To IgA level. Conclusion　The expression of CD25 in peripheral blood T cells and the level of serum CD25 are closely related to the developrnent and renal involvement of children with HSP，which is helpful for the evaluation of the disease.

Key words: Henoch-Schonlein purpura, Henoch-Schonlein purpura nephritis, CD25, SIL-2R, T cell

摘要： 目的　探讨外周血T细胞表面和血清CD25表达水平与儿童过敏性紫癜（HSP）并发肾炎的相关性，为HSP病情的评估提供帮助。方法　收集中国医科大学附属第一医院2020年1月至2021年11月收治的HSP患儿40例作为研究组，根据肾脏受累情况再分为紫癜性肾炎（HSPN，18例）组与非紫癜性肾炎（NHSPN，22例）组。同期健康体检儿童25名作为对照组（HD）。采用流式细胞仪检测外周血T细胞中CD25的表达。酶联反应吸附（ELISA）法测定血清CD25水平。比较组间差异及相关性。结果　与对照组相比，HSP患儿外周血中CD25在CD3+总T细胞［HD（2.19±0.85）%、HSP（1.43±0.78）%］、CD3+CD4+T细胞［HD（3.82±2.18）% 、HSP（2.40±0.95）%］、CD3+CD4-T细胞［HD（1.90±0.51）%、HSP（1.25±0.35）%］上的表达均减少（P<0.05）。HSPN患儿外周血CD3+CD4+T细胞中CD25的表达［NHSPN（2.62±1.03）% 、HSPN（2.11±0.78）%］和血清CD25水平［NHSPN（649.0±346.5）pg/mL、HSPN（407.0±141.0）pg/mL］较NHSPN患儿下降（P<0.05）。HSP患儿血清CD25水平与IgA水平呈负相关（r=-0.5060，P<0.05），而外周血T细胞中CD25的表达与血清IgA水平相关性差异无统计学意义。结论　外周血T细胞CD25的表达和血清CD25的水平与HSP疾病发生和肾脏受累情况相关，有助于疾病的评估。

关键词: 过敏性紫癜, 紫癜性肾炎, CD25, 血清可溶性白细胞介素-2受体, T细胞