Abstract:

Objective    To investigate the expression of multidrug resistant-associated protein 1 in the peripheral blood of children with refracotory epilepsy. Methods    The expression of MRP1 was detected by RT-PCR and Western blot in children with intractable epilepsy，epilepsy controlled by anti-epileptic drugs （AEDs） and healthy children.Results    The two methods showed that MRP1 expression in intractable epilepsy was higher than that in epilepsy controlled by anti-epileptic drugs （AEDs） and healthy children（P＜0.05）， and there was no difference between epilepsy controlled by anti-epileptic drugs （AEDs） and healthy children.Conclusion    MRP1 overexpresses in peripheral blood of children with refractory epilepsy.

Key words: refactory epilepsy, multidrug resistant-associated protein 1, children, peripheral blood

摘要：

目的    研究儿童难治性癫痫外周血多药耐药相关蛋白1（MRP1）的表达情况。方法    2010年11月至2011年10月徐州市儿童医院门诊及住院收治的癫痫患儿60例，分为难治性癫痫组和药物控制良好组，各30例；选取同期来院体检的健康儿童30名为对照组。采用逆转录酶聚合酶链式反应（RT-PCR）和蛋白免疫印迹（Western blot）方法测定3组外周血MRP1 mRNA及蛋白含量。结果    难治性癫痫组MRP1表达明显高于药物控制良好癫痫组及对照组，差异均有统计学意义（P＜0.05）；药物控制良好癫痫组与对照组比较差异无统计学意义（P＞0.05）。结论    难治性癫痫患儿外周血MRP1表达增强。

关键词: 难治性癫痫, 多药耐药相关蛋白1, 儿童, 外周血