Abstract:

Abstract：Objective    To  study the  aetiological features of  hand-foot-mouth  disease and the genetic characteristic of EV7 l in Guangzhou in 2011. Mehtods    Totally 63 blood，pharynx swab and stool samples of hand-foot-mouth disease were collected from Guangzhou Women and Children Medical Center in 2011.Twelve EV7l positive samples were used to isolate  the  virus and  EV7l strains’VPl  were sequenced and phyletic evolution was analysed. Results  The affinities of 12 clinical isolates of Guangzhou area in 2011were far from A， B subtype， and the affinities of nucleotide homology and amino acid homology were less than 85% and 96.5%. With the C subtype genetic type being relatively close， nucleotide， amino acid homology over 88.5% and 97.5%， especially with the C4 subtype affinities of recently， the nucleotide and amino acid homology were 92.9% ~ 94.6% and 97.9% ~ 98.9%. Conclusions    Totally 12 EV71 isolates strains of Guangzhou area in 2011 belong to subtype C4a in the evolutionary process.

Key words: hand-foot-mouth disease, enterovirus71, vival structural proteins, phylogeny

摘要：

摘要:目的    研究广州地区2011年手足口病病原流行特点及EV71的基因特征。方法    收集2011年1~11月广州市妇女儿童研究中心63份不同患者来源的肛拭子、咽拭子以及血液样本，选择12份EV71阳性标本进行病毒分离，扩增EV71VP1区，测序并与EV71各血清型代表株序列比对，进行进化分析。结果    2011年广州地区12个临床分离株同Ａ、Ｂ亚型的亲缘性较远，核苷酸同源性都不足85%，氨基酸同源性不足96.5%。其同Ｃ亚型的亲缘型较近，核苷酸、氨基酸同源性超过88.5%和97.5%，尤其与 C4亚型的亲缘性最近，核苷酸和氨基酸的同源性分别为 92．9%～ 94.6 %和 97.9% ～98.9 %。结论    2011年广州地区的12株EV71分离株都属于C4a亚型进化分支，并处于选择进化过程中。

关键词: 手足口病, 肠道病毒71, 种系发生, 病毒结构蛋白质类