Abstract:

Abstract： Objective　To study the level of the IL-2 and IL-6 in serum and induced sputum in the children with mycoplasma pneumoniae pneumonia，then approach the role and clinical significance in the nosogenesis of the mycoplasma pneumoniae pneumonia. Methods　Fifty-six children with mycoplasma pneumoniae pneumonia were collected from June 2009 to December 2010 in our hospital and the mass concentration of the IL-2 and IL-6 from fifty-six serum and forty-two induced sputum specimens was detected with enzyme linked immunosorbent assay，then the level of the IL-2 and IL-6 in serum and induced sputum were compared in different stages and mild and severe case with t-test. Results　The level of the IL-2 of the children with mycoplasma pneumoniae pneumonia in serum and induced sputum in acute stage was lower than the convalescence stage and normal control group’s（P < 0.05）. The level of the IL-6 was higher than the convalescence stage and normal control group’s（P < 0.05）. The level of the IL-2 of the mild case with mycoplasma pneumoniae pneumonia in acute and convalescence stage was higher than the severe case’s（P < 0.05）. The level of the IL-6 of the mild case was lower than the severe case’s（P < 0.05）. The detection results of the two specimens from serum and induced sputum were accordant and the level of the IL-2 and IL-6 in induced sputum was higher than the level of the serum group，both positively related. Conclusion　The level of the IL-2 and IL-6 in serum and induced sputum could be detected in infection incipient stage with mycoplasma pneumoniae so that the severity level and prognosis of the pathogenetic condition could be evaluated and judged.Meanwhile the evidences could be provided for the earlier immunologic intervention treatment.

Key words: mycoplasma pneumoniae, pneumonia, IL-2, IL-6

摘要：

目的　研究肺炎支原体肺炎（MPP）患儿血清及诱导痰中白细胞介素2（IL-2）和白细胞介素6（IL-6）的表达，探讨其在MPP感染发病机制中的作用及临床意义。方法　收集华中科技大学同济医学院附属孝感医院儿科2009年6月至2010年12月住院确诊的56例MPP患儿，采用酶联免疫吸附实验（ELISA法）检测56例患儿血清及42例患儿诱导痰中IL-2和IL-6的质量浓度，用t检验比较患儿血清及诱导痰中IL-2、IL-6在不同病期和轻重症中的表达水平。以同期40名体检的儿童作为正常对照组。结果　MPP患儿血清及诱导痰中IL-2在急性期明显低于恢复期和正常对照组（P < 0.05），IL-6在急性期明显高于恢复期和正常对照组（P < 0.05）；MPP患儿血清及诱导痰中IL-2在急性期和恢复期中轻症组均高于重症组（P < 0.05），IL-6在急性期和恢复期中轻症组均低于重症组（P < 0.05）；血清和诱导痰中两种标本检测结果一致，且诱导痰中IL-2和IL-6总体上高于血清组，两者呈正相关。结论　IL-2和IL-6在MP感染发病机制中起重要作用。MP感染初期可通过检测血及诱导痰中的IL-2和IL-6，为病情严重程度及预后作出深入评估和判断，同时提供早期进行免疫干预治疗的依据。

关键词: 肺炎支原体, 肺炎, 白细胞介素2, 白细胞介素6