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QIAN Yi,FENG Qi-hua.
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钱 毅1,封其华2
Abstract: Objective To observe the changes of the functional states of lymphocyte subgroup and immunoglobulin level of children with Henoch -Schonlein(HSP)in acute phase, and to evaluate its mechanisms in the pathogenesis of HSP and their clinical significance. Methods Forty HSP patients were divided into the group of Henoch-Schonlein purpura nephritis(HSPN)and the group of non-HSPN. The control group consisted of 20 healthy children. The plasma immunoglobulin IgA、 IgG and IgM were detected with nephelometer and lymphocyte subgroup CD3+、CD4+、CD8+ 、CD4+/CD8+、CD19+、CD19+23+、CD16+CD56+、CD4+/CD8+ and CD4+CD25+cell were detected with flow cytometry. Results The contents of CD3+、CD4+ 、CD4+CD25+、CD16+CD56+ cell and the ratio of group CD4+/CD8+ cell in HSP group were significantly lower than that in control group (P < 0.01).The contents of CD8+、CD19+、CD19+23+ cell in HSP group were significantly higher than that in control group(P < 0.01). There was also no significant difference in the levels of lymphocyte subgroup between HSPN group and control group(P > 0.05). However, the levels of IgA in HSPN group was significantly higher than that in control group(P < 0.01). There was no significant difference in the levels of IgG and IgM between non-HSP group and control group (P > 0.05).There was significant difference in the levels of IgG (P <0.05),and no significant difference between HSPN and non-HSPN(P > 0.05). Conclusion The results show that there is a polyclonal B cell activation characterized by significantly increased IgA in HSPN and NHSPN, which provides objective index and theoretical evidences for dignosis and treatment of HSP.
Key words: immunoglobulin , children, Lymphocyte subgroup
摘要: 研究儿童过敏性紫癜(HSP)急性期免疫球蛋白与淋巴细胞亚群的变化,探讨其临床意义。方法 研究对象为2006年1月至2007年8月在苏州大学附属 儿童医院住院的HSP急性期患儿40例,根据临床有无肾脏损害分为肾炎(HSPN)组和非肾炎(NHSPN)组,各20例,并以20名正常儿童作为对照组,采用速率散射 比浊法测定免疫球蛋白IgA、IgG、IgM,采用流式细胞仪分析技术检测外周血单个核细胞CD3+、CD4+、CD8+、CD4+/CD8+、CD19+、CD19+23+、CD16+56+、 CD4+CD25+等淋巴细胞亚群的水平。结果 HSPN组与NHSPN组外周血单个核细胞CD3+、CD4+、CD4+/CD8+、CD4+CD25+、CD16+56+水平明显下降, 两组分别与正常对 照组比较差异有统计学意义(P < 0.01);CD8+、CD19+、CD19+23+水平明显上升,两组分别与正常对照组比较差异有统计学意义(P < 0.01);HSPN组与NHSPN 组淋巴细胞亚群组间对比无统计学意义(P > 0.05);血清免疫球蛋白IgA明显上升,HSPN组与NHSPN组分别与正常对照组比较差异有统计学意义(P < 0.01); NHSPN组IgG、IgM变化不大,与对照组比较差异无统计学意义(P > 0.05);HSPN组IgG降低与对照组比较差异有统计学意义(P < 0.05),IgM变化不大,与对照 组比较差异无统计学意义(P > 0.05)。结论 HSP患儿急性期B淋巴细胞呈多克隆活化,抗体分泌增多,这为临床采取相应的免疫调节治疗提供了理论依据。
关键词: 过敏性紫癜, 儿童, 淋巴细胞亚群, 免疫球蛋白
QIAN Yi,FENG Qi-hua.. Research on immune state of children with Henoch-Schonleinin acute in phase.[J]. .
钱毅,封其华. 儿童过敏性紫癜急性期免疫状态研究[J]. 中国实用儿科杂志.
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