Abstract: AbstractObjectiveTo analyze the clinical manifestation of newborns with infection caused by staphylococcus aureus (S. aureus) and investigate the antibioticresistance phenotype of these strains. MethodsS. aureus isolates were identified with GPI card of Vitek system. Antibiotics sensitivity test were performed by using disk diffusion methods,and sensitivity test of oxacillin and vancomycin was performed with a further Etest method.The mecA gene was detected with PCR. ResultsThirtyfive neonates (21 males and 14 females) with infection caused by staphylococcus aureus were identified, aged from 6d to 28d,including 24 cases of pneumonia, 7 cases of septicaemia, 2 cases of hypodermic gangrene, 1 case of phlegmona with formation of an abscess and 1 case of omphalitis. Drug susceptibility test showed that 97.1% of 35 strains were βlactamasepositive and resistant to penicillin, while 82.9% of all strains were sensitive to oxacillin with MIC50 0.5 μg/m and MIC90 4.0 μg/mL. All strains were sensitive to vacomycin and rifampin , while 82.9% of all strains were susceptible to cefotaxime and ceftriaxone. Resistance rates to erythromycin, tetracycline, clindamycin, trimethoprimsulfamethoxazole, ofloxacin and chloramphenicol were 54.3%, 37.1%, 17.1%, 14.3%, 5.7% and 2.9% respectively.The mecAPCR showed that the control strain ATCC25923 and all oxacillinsensitive S. aureus were mecAnegative ,while 6 oxacillinresistant strains were mecApositive. ConclusionLow respiratory tract infection is the most common disease in all infections caused by S. aureus in newborn children and most of them are community acquired.