中国实用儿科杂志

中国实用儿科杂志

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小儿幽门螺杆菌感染诊断方法的比较研究

苏林，刘伟，张寅，奚容平，许春娣   

  1. 上海交通大学医学院附属瑞金医院儿内科，上海200025
  • 收稿日期:2006-01-12 修回日期:2006-06-15 出版日期:2006-09-06 发布日期:2006-09-06

Comparing the ASSURETM RAPID test with other diagnostic methods for helicobacter pyloric infection in children

Su Lin,Liu Wei,Zhang Yin,et al.   

  1. Department of Pediatrics, Rujin Hospital, Shanghai Jiao Tong University,Shanghai 200025,China
  • Received:2006-01-12 Revised:2006-06-15 Online:2006-09-06 Published:2006-09-06

摘要： 目的比较儿科消化专业临床常用的几种H·pylori诊断方法的准确性，并对新的快速血液学诊断法作考核和评价。 方法对200412—200503上海交通大学医学院附属瑞金医院儿内消化专科门诊100例3~16岁患儿做胃镜检查、13CUBT、胃镜检查时在胃窦部取黏膜组织作尿素酶试验、组织学检查，并同时抽血做H·pylori抗体测定。 结果100例患儿中，55例感染H·pylori(感染率为55%)，尿素酶、组织学、13CUBT、血金标法的敏感性分别为：81.48%、75.93%、96.30%、94.50%，特异性为86.96%、80.43%、100%、91.11%。金标法血清与全血测定结果显示敏感性、特异性几乎相同。 结论尿素酶试验和组织学检查能直接检测胃黏膜中的H·pylori，有较高的敏感性与特异性，但需经胃镜取胃黏膜才能完成。13CUBT能反映全部胃黏膜的感染情况，敏感性、特异性最高，是目前临床诊断的金标准之一，且非侵入性。血液金标法检测，操作方便，仅需1滴血，便可反映出H·pylori的感染状况，可作为H·pylori的流行病学检查及筛查。

Abstract: AbstractObjectiveIn this study,we compared the ASSURETM RAPID test,a serological assay for antiH·pyloric antibodies,with severl methods which are commonly used in pediatrics. MethodsOur study has recuited 100 patients (age 3~16 years old, 47 male, and 53 female) with upper gastrointestinal symptoms. We tested them for H·pyloric with rapid urease test(CLO test),histological examination of gastric biopsy sample, and anti-H.pyloric antibodies. ResultsThe sensitivities,CLO test, histological examination, 13Curease breath test and ASSURETM RAPID test,were81.48%,75.39%,96.3%,94.50%.specificities,were86.96%,80.43%100%,91.11%, respectively. ConclusionFurthermore, ASSURETM RAPID test, was equally effective with either whole blood or serum samples. With the convenience in its administration and high degree of sensitivity and specificity, we concluded that ASSURETM RAPID test is highly desirable for studying the epidemiology of H.pyloric infections where medical facilities and finacial resources are limited.