中国实用儿科杂志

中国实用儿科杂志

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反义寡核苷酸对激素敏感型单纯性肾病患儿外周血单个核细胞病毒基因表达的抑制作用

刘晓鸣,王峥,朱小石   

  1. 四川大学华西第二医院，四川成都610041
  • 收稿日期:2006-02-01 修回日期:2006-06-12 出版日期:2006-10-06 发布日期:2006-10-06

Inhibition of the gene expression of respirovirus in steroid responsive simple nephrotic syndrome by antisense oligonucleotides.

Liu Xiaoming,Wang Zheng,Zhu Xiaoshi.   

  1. The Second West China Hospital,Sichuan University,Chengdu 610041,China
  • Received:2006-02-01 Revised:2006-06-12 Online:2006-10-06 Published:2006-10-06

摘要： 目的通过设计反义寡核苷酸(ASONs)抑制激素敏感型单纯性肾病(SRSNS)呼吸道病毒基因的表达，提供激素敏感型单纯性肾病呼吸道病毒感染的证据。 方法将ASONs转染至外周血单个核细胞(PBMC)后，通过RTPCR的方法检测呼吸道病毒基因的表达。 结果SRSNS活动期PBMC中呼吸道病毒基因的表达能被其相应的ASONs所抑制。呼吸道合胞病毒基因表达受抑制率为93.9%(14/15)，流感病毒受抑制率为87.5%(7/8)，总抑制率为91.3%(21/23)。 结论呼吸道病毒确实存在于SRSNS活动期病人体内，呼吸道病毒感染作为主要触发因素，可能为SRSNS的重要发病机制。

关键词: 反义寡核苷酸, 呼吸道病毒, 激素敏感型单纯性肾病综合征

Abstract: AbstractObjectiveTo study the inhibition of antisense oligonucleotides (ASONs) on the gene expression of respiroviruses in peripheral blood mononuclear cells(PBMCs)of children with steroid responsive simple nephrotic syndrome (SRSNS),aiming to provide more proof for the infection of respiroviruses in SRSNS. MethodsAfter transfecting ASONs to PBMCs of children with SRSNS,the gene expression of respiroviruses was detected by RTPCR. ResultsAfter ASONs transfection,the gene expression of respiroviruses was inhibited significantly in SRSNS(active stage). ConclusionThe study demonstrates that respiroviruses infection actually exists in SRSNS(active stage),and the gene expression of the respiroviruses is inhibited by ASONs,thus suggesting that respiroviruses infection may be one of the triggers of SRSNS.

Key words: Respiroviruses, Steroid responsive simple nephrotic syndrome