中国实用儿科杂志

中国实用儿科杂志

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白三烯D4对白细胞介素4诱导嗜酸性粒细胞趋化因子-3在支气管上皮细胞表达的影响

欧维琳a，朱春江a，黄健b，韦欢a   

  1. 桂林医学院 a附属医院儿科 b基础学院，广西桂林541001
  • 收稿日期:2006-10-12 修回日期:2007-01-05 出版日期:2007-04-06 发布日期:2007-04-06

Impact of leukotriene D4 on the expression of eotaxin-3 induced by interleukin-4 in bronchial epithelial cell.

OU Wei-lin*,ZHU Chun-jiang,HUANG Jian,et al.   

  1. *Department of Pediatrics,Guilin Medical College Affiliated Hospital,Guilin 541001,China.
  • Received:2006-10-12 Revised:2007-01-05 Online:2007-04-06 Published:2007-04-06

摘要： 目的研究白三烯D4是否参与调节支气管上皮细胞表达嗜酸性粒细胞趋化因子-3(eotaxin-3，Eot-3)，探讨白三烯和支气管上皮细胞在气道变态 反应性炎症中的作用。 方法用白三烯D4预处理支气管上皮细胞1h，然后用白细胞介素-4(IL-4)刺激细胞24h，RT-PCR法检测Eot-3在mRNA水平的表达；ELISA法检测细胞 培养上清液中Eot-3蛋白的表达。 结果未经处理的支气管上皮细胞仅表达微量的Eot-3，用IL-4刺激细胞并培养24h后，在mRAN和蛋白水平的Eot-3表达明显增强，白三烯D4预处理 细胞1h，可以使IL-4的这种诱导作用加强。 结论支气管上皮细胞不仅是一种屏障细胞，也是一种效应细胞，在炎症介质的刺激下能表达Eot-3，参与支气管炎症的产生。白三烯D4通过上调 IL-4诱导Eot-3在上皮细胞的表达可能是其参与支气管哮喘嗜酸性粒细胞炎症的机制之一。

关键词: 支气管上皮细胞, 白三烯, 白介素-4, 嗜酸性粒细胞趋化因子-3

Abstract: AbstractObjectiveTo investigate whether leukotriene D4 regulates the expression of eotaxin-3 in bronchial epithelial cells.To explore the role of leukotriene D4 and bronchial epithelial cells on airway allergic inflammation. MethodsPretreated BEAS-2B cells and NHBE cells with leukotriene D4 for 1 hour，stimulated with interleukin-4,the cells were incubated for 24 hours.The messenger RNA (mRNA) of eotaxin-3 was tested by reverse transcription polymerase chain reaction (RT-PCR) and the eotaxin-3 protein was determined by ELISA. ResultsThe untreated bronchial epithelial cell expressed eotaxin-3 on a very low level.After being stimulated with IL-4 and incubated for 24h,the eotaxin-3 production increased significantly both on mRNA and protein level.Pretreating the cells with leukotriene D4 could enhance the inducing effect of IL-4. ConclusionBronchial epithelial cells are not only the barrier of airway,but also a kind of response cell.It can express eotaxin-3 and involve the airway allergic inflammation.Leukotreine D4 can up-regulate the expression of eotaxin-3 induced by IL-4 on bronchial epithelial cells.It may explain partially the mechanism of leukotrienes involving airway allergic inflammation of asthma.

Key words: Eotaxin-3 , Leukotrienes, Interleukin-4