中国实用儿科杂志

中国实用儿科杂志

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细胞周期相关因子cdt1在儿童急性淋巴细胞白血病中的表达

文川，万伍卿，刘瑛，刘晓艳，田继东，邓兵   

  1. 中南大学湘雅二医院儿科，湖南长沙410011
  • 收稿日期:2006-07-28 修回日期:2006-10-08 出版日期:2007-02-06 发布日期:2007-02-06

Expression of cellcycle associated factor cdt1 in children with acute lymphoblastic leukemia.

WEN Chuan,WAN Wuqing,LIU Ying,et al.   

  1. Department of Pediatrics，the Second Xiangya Hospital，Central South University 410011,China
  • Received:2006-07-28 Revised:2006-10-08 Online:2007-02-06 Published:2007-02-06

摘要： 目的研究儿童急性淋巴细胞白血病中细胞周期相关因子cdt1蛋白及mRNA的表达情况。 方法200508—200603中南大学湘雅二院儿科以16例首次诊断且未治疗的急性淋巴细胞白血病患儿为实验组，以16例正常儿童为对照组，采集其静脉血进行淋巴细胞分离，采用免疫组织化学染色法检测cdt1蛋白在2组中的表达，采用RTPCR法检测cdt1 mRNA在2组中的表达情况。采用SPSS11.5软件进行数据分析。 结果实验组cdt1蛋白表达高于对照组；实验组cdt1 mRNA表达高于对照组；对照组cdt1蛋白有表达，但其相应的mRNA不表达。 结论急性淋巴细胞性白血病细胞cdt1过度表达，可能参与儿童急性淋巴细胞白血病的发生发展；正常淋巴细胞cdt1存在蛋白与其相应的mRNA表达不一致的现象。

关键词: 细胞周期, cdt1, 白血病, 淋巴细胞, 急性, 儿童

Abstract: AbstractObjectiveTo research the expression of cellcycle associated factor cdt1 in children with acute lymphoblastic leukemia. MethodsSixteen children with acute lymphoblastic leukemia were firstly dianosed and were not treated(research group) and 16 healthy children(control group) whose intravenous blood was collected for isolating the singlenucleus cell were controls.The expression of cdt1 in children was measured by immunohistochemistry for protein,and by reverse transcriptionpolymerase chain reaction(RTPCR) for their mRNA.The data was analyzed by using software SPSS11.5 for windows. ResultsThe expression of cdt1 proteins in research group was obviously higher than those in control group(P<0.05);the expression of cdt1 mRNA in research group was obviously higher than those in control group(P<0.05);the cdt1 proteins were expressed,but the expression levels of their corresponding mRNA were not measured in control group. ConclusionCdt1 is related to cell cycle.The expression of cdt1 will result in the development of acute lymphoblastic leukemia;the cdt1 proteins are not related to the expression level of the corresponding mRNA in control group.

Key words: Children , Cdt1, Leukemia，Lymphocyte，Acute