中国实用儿科杂志

中国实用儿科杂志

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金黄色葡萄球菌对苯唑西林的耐药性研究和mecA基因检测

杨荣旺1，华春珍2，毛淑炯3，李珊2   

  1. 1.浙江大学医学院附属儿童医院，浙江杭州310003；2.杭州师范学院附属医院，浙江杭州310018；3.杭州师范学院临床医学院，浙江杭州310018
  • 收稿日期:2006-03-20 修回日期:2006-06-11 出版日期:2006-09-06 发布日期:2006-09-06

Study on oxacillinresistance of staphylococcus aureus and detection of mecA gene.

Yang Rongwang*,Hua Chunzhen,Mao Shujiong,et al.   

  1. *The Children's Hospital,Zhejiang University School of Medicine,Hangzhou 310003,China
  • Received:2006-03-20 Revised:2006-06-11 Online:2006-09-06 Published:2006-09-06

摘要： 目的了解杭州地区金黄色葡萄球菌(简称金葡菌)对苯唑西林的耐药情况，探索PCR法检测耐药性相关的mecA基因的敏感性和特异性。 方法用金葡菌乳胶凝集试验和Vitek系统GPI卡鉴定金葡菌，用纸片扩散法和Etest法进行药敏试验，并对所有菌株均采用PCR技术检测与耐药性相关的mecA基因。 结果KirbyBauer法和Etest法检测金黄色葡萄球菌对苯唑西林的敏感性，133株中敏感株分别为113株(85.0%)和115株(86.5%)，中介株分别为2株(1.5%)和4株(3.0%)，耐药率(株)分别是18株(13.5%)和14株(10.5%)；所有菌株均对万古霉素敏感。mecA基因的PCR扩增结果显示：所有的苯唑西林敏感株mecA基因均阴性，中介株mecA基因阳性1株，而所有耐药株均阳性，标准株ATCC25923则为阴性。杭州地区金葡菌对苯唑西林的耐药率为10.5%，以Etest法为金标准，纸片扩散法和PCR法的耐药菌检出的敏感性为100%，而特异性分别为96.6%和98.3%。 结论金葡菌对苯唑西林的耐药率不高，而mecAPCR技术可以作为快速检测耐苯唑西林金葡菌的有效方法。

关键词: 金黄色葡萄球菌, mecA基因MRSA, PCR, 耐药性

Abstract: AbstractObjectiveTo study the antimicrobial agents resistance of staphylococcus aureus in Hangzhou area and the sensitivity and specificity of the mecA gene PCR assay. MethodsA total of 133 staphylococci strains were isolated from the children in Hangzhou,then Disc Diffusion and staphylococcal mecA gene PCR assay for detecting MRSA were compared with Etest method. ResultsOf all 133 strains performed with both KirbyBauer and Etest methods in the susceptibility test,85.0% and 86.5% of the isolates were susceptible to oxacillin respectively.All of the isolates were susceptible to vancomycin with Etest MICs ≤4.0 ug/ml,and 91.7% of them had the zone diameters not more than 15mm.The sensitivity of Disc Diffusion and mecA gene PCR assay for detecting MRSA were 100% respectively,the Disc Diffusion specificity was 96.6%,and the mecA gene PCR assay's specificity was up to 98.3%. ConclusionRate of oxacillin resistance staphylococcus aureus is low in the study,and mecA gene PCR assay is significant in rapid detection of MRSA.

Key words: Drug resistance , MecA gene, MRSA polymerasechain reaction