中国实用儿科杂志

中国实用儿科杂志

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干扰素γ加强肿瘤坏死因子相关凋亡诱导配体抑制神经母细胞瘤细胞的作用

李爱敏1,李春艳2,于惠芹1,初青1,孙洪亮1   

  1. 1.青岛大学医学院附属烟台毓磺顶医院儿科,山东烟台 264000；2.中国医科大学实验技术中心三部，辽宁沈阳 110001
  • 收稿日期:2006-01-09 修回日期:2006-03-22 出版日期:2006-06-06 发布日期:2006-06-06

IFNγ enhance the ability of the inhibition of TRAIL to neuroblastoma cell line.

Li Aimin*,Zhang Ying,Yu Huiqin,et al.   

  1. *Yuhuangding Hospital,Yan Tai 264000,China
  • Received:2006-01-09 Revised:2006-03-22 Online:2006-06-06 Published:2006-06-06

摘要： 目的探讨肿瘤坏死因子相关凋亡诱导配体(TRAIL)对人神经母细胞瘤的作用及干扰素γ(IFNγ)对新型凋亡分子TRAIL抗瘤活性的影响，并探讨其影响机制。 方法应用MTT分析和流式细胞仪研究TRAIL对人神经母细胞瘤细胞的抑制作用及IFNγ对TRAIL抗瘤活性的影响。用RTPCR方法检测IFNγ作用48h后，Caspase8mRNA的表达。 结果TRAIL(10,50,100μg/L)单独作用、IFNγ(1000U/mL)单独作用后，对SY5Y细胞的增殖抑制率分别为(438±089)%、(354±166)%、(503±126)%、(533±206)%；凋亡率分別为(518±333)%、(526±364)%、(500±288)%、(814±735)%。IFNγ(1000U/mL)与TRAIL(100μg/L)联合作用后，对SY5Y细胞的增殖抑制率为(4122±709)%；凋亡率为(2129±620)%。RTPCR方法发现IFNγ作用48h后Caspase8mRNA的表达明显上调。 结论神经母细胞瘤细胞SHSY5Y对TRAIL不敏感，IFNγ可以明显提高TRAIL对神经母细胞瘤细胞的敏感性，其发生机制可能是通过IFNγ上调Caspase8mRNA的表达而实现的。

关键词: 神经母细胞瘤, IFNγ, 肿瘤坏死因子相关凋亡诱导配体, Caspase8

Abstract: AbstractObjectiveTo explore the effect of TRAIL on neuroblastoma cell line and the influence of IFNγ on the antitumor effects of TRAIL. MethodsUse MTT and flow cytometric analysis to detect the antitumor effects of TRAIL and study the effects of IFNγ on TRAIL in inhibiting human neuroblastoma cells. ResultsThe rates of killing of TRAIL alone、IFNγ alone and the combination of TRAIL and IFNγ were (503±126)%、(533±206)%、(4122±709)%,IFNγ could enhance the killing rate of TRAIL to the SY5Y cells.FCM analysis suggested that IFNγ enhanced the killing rate of TRAIL to the SY5Y cells based on inhibiting apoptosis of SY5Y cells. ConclusionSHSY5Y cells are not sensitive to TRAIL.IFNγ can upregulate the apoptosis of SY5Y induced by TRAIL.

Key words: TRAIL, Caspase8 , IFNγ