中国实用儿科杂志

中国实用儿科杂志

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肥胖儿童载脂蛋白E基因型与临床表现的相关性

刘长云1,管立学2,王永芹1,季加芬1,刘红英1,王敬先2,李伟华1,别惠玲1,孙未1   

  1. 1山东省潍坊医学院附属医院儿科(261031),2潍坊市人民医院遗传室(261041)
  • 收稿日期:2005-03-10 修回日期:2005-09-15 出版日期:2005-12-25 发布日期:2005-12-25

Clinical studies on the investigation of Apo E polymorphism in children with obesity.

Liu Changyun*，Guan Lixue,Wang Yongqin,et al.   

  1. *The Hospital Affiliated to Weifang Medical College,Weifang 261031，China
  • Received:2005-03-10 Revised:2005-09-15 Online:2005-12-25 Published:2005-12-25

摘要： 目的探讨单纯性肥胖儿童载脂蛋白(apolipoprotein,Apo)E基因多态性的分布及其对血脂、脂蛋白、载脂蛋白的影响，及其与冠状动脉、心电图改变的关系。并对其早期预测和疾病预防提供理论依据。 方法选择2002年12月至2004年12月潍坊医学院附属医院儿科的6~14岁单纯性肥胖儿童89例和健康儿童76例。抽取外周静脉血，测定血清中甘油三酯(TG)，总胆固醇(TC)，高密度脂蛋白胆固醇(HDLC)，低密度脂蛋白胆固醇(LDLC)，载脂蛋白A1(Apo A1)，载脂蛋白B100(Apo B100)浓度。应用改良的聚合酶链式反应限制性片段长度多态性(PCRRFLP)分析及聚丙烯酰胺凝胶电泳测定儿童Apo E基因型。 结果共检出4种Apo E基因型，E3/3、E4/3、E2/3、E4/2，以ε3为最常见。与健康儿童比较，肥胖儿童ε4等位基因频率增高，差异有显著性(P<005)。 结论单纯性肥胖儿童有Apo E基因多态性的变化，且明显影响小儿血浆脂类代谢，肥胖儿童Apo E4与冠心病有密切相关性。

关键词: 胆固醇, 载脂蛋白Ｅ, 等位基因, 肥胖症, 儿童

Abstract: AbstractObjectiveTo investigate the distribution of apolipoprotein(Apo)E polymorphism and association of Apo E genotypes with plasma lipids in children with simple obesity.And to provide a theoretical basis for the prevention of CHD. MethodsFrom Dec.2002 to Dec.2004,89 children with simple obesity and 76 healthy children from 6 to 14 years old were chosen as obesity group and control group.Serum triglyceride(TG)，total cholesterol(TC)，low density lipoprotein cholesterol(LDLC)，high density lipoprotein cholesterol(HDLC)，Apolipoprotein A1(Apo A1)，Apolipoprotein B100(Apo B100)were measured after they were fasted for 12 hours.Polymerase chain reactionrestricted fragments length polymorphism(PCRRFLP)，polyacrylamide gel electrophoresis，and silver staining technique were used for the detection of apolipoprotein E genotype. ResultsFour genotypes of Apo E were detected,and their distribution was imbalanced，most of them being ε3 alleles.Compared with the control group，the frequency of ε4 allele in obesity group was higher(P<005).Apo E polymorphisms may affect plasma lipid levels of healthy and obesity children. ConclusionChildren with the simple obesity have different patterns of Apo E polymorphisms as compared with healthy children.Apo E polymorphisms may affect plasma lipids metabolism.

Key words: Obesity, Children , Apolipoprotein E, Alleles