中国实用儿科杂志

中国实用儿科杂志

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肺炎支原体肺炎患儿血清及诱导痰中白细胞介素-4及γ-干扰素的测定

王俊1,韩晓华1,杨柏松2,尚云晓1,刘春峰1,蔡栩栩1   

  1. 1中国医科大学附属二院儿科(沈阳，110003)，第一作者现在沈阳市第四人民医院儿科 (110031) 2解放军第463医院(沈阳，110042)
  • 收稿日期:2005-03-19 修回日期:2005-06-30 出版日期:2005-09-25 发布日期:2005-09-25

Determination of serum and sputum IL-4,IFN-γ levels in children with mycoplasma pneumoniae pneumonia.

Wang Jun,Han Xiaohua,Yang Baisong,et al.   

  1. Department of Pediatrics,the Second Hospital Affiliated to China Medical University,Shenyang 110003,China
  • Received:2005-03-19 Revised:2005-06-30 Online:2005-09-25 Published:2005-09-25

摘要： 目的研究肺炎支原体肺炎(MPP)患儿血清及诱导痰中白细胞介素-4(IL-4)及γ-干扰素(IFN-γ)质量浓度变化，探讨其在MPP发病机制中的作用。 方法2003年2~8月对中国医科大学附属二院儿科住院的MPP患儿38例，用ELISA法测定38例血清及24例诱导痰中急性期及恢复期的IL-4及IFN-γ质量浓度。 结果MPP患儿血清及诱导痰IL-4质量浓度急性期明显高于恢复期和正常对照组(P<0.01);IL-4/IFN-γ比值急性期高于恢复期和正常对照组(P<0.05)。MPP患儿急性期血清及诱导痰IL-4质量浓度重症组明显高于轻症组(P<0.01),急性期血清及诱导痰IL-4/IFN-γ比值重症组高于轻症组(P<0.05);诱导痰与血清两种标本检测结果一致，诱导痰中的质量浓度高于血清中的质量浓度，两者呈正相关。 结论MPP患儿存在TH1/TH2功能紊乱，MPP患儿TH2反应占优势，IL-4及IFN-γ参与MPP患儿的免疫状态改变，在MPP发病机制中发挥一定作用。

关键词: 肺炎支原体肺炎, 白细胞介素-4, γ-干扰素

Abstract: To study the contribution of IL-4,IFN-γ in serum and in induced sputum in children with MPP. MethodsThe IL-4 and IFN- γ levels were determined in 38 serum and 24 induced sputum using an ELISA kit in the acute episode and remission period in MPP children. ResultsThe IL-4 levels in the serum and induced sputum were significantly higher in the acute episode period in MPP compared with children in remission period and the controls(P<0.01).The IL-4/IFN-γ ratios in the acute episode period were higher than that in remission period and the controls(P<0.05).The IL-4 levels in the serum and induced sputum were significantly higher in severe patients than mild patients with MPP ( P<0.01).The serum and induced sputum IL-4/IFN-γ ratios in severe patients were higher than that of mild patients(P<0.05).The serum was in accordance with sputum in two kinds of specimens examination result.The cytokines density in sputum were higher than that of serum,both positively related. ConclusionThe data of serum and sputum cytokine suggest that the imbalance of TH1/TH2 exists in the MPP children.It seems to represent a predominant TH2-like cytokine response in MPP.The IL-4 and IFN-γ may play a significant role in the immunopathologic responses in MPP.

Key words: IFN-γ , IL-4