Abstract: Objective　To study the effects of quercetin on the biological behavior of human gingival fibroblasts（HGFs）stimulated by Porphyromonas gingivalis lipopolysaccharide（P.g LPS）. Methods　DNA fragment electrophoresis，CCK-8 and wound healing method were used to observe the effect of different concentrations（10，20，50，100 μmol/L）of quercetin on cell toxicity，cell proliferation，and cell migration of HGFs. Then we further stimulated HGFs with P.g LPS to create an in vitro inflammatory model；by applying flow cytometry，reactive oxygen species（ROS）test，enzyme-linked immunosorbent assay（ELISA）and realtime quantitative PCR（qRT-PCR），we tested the influence of quercetin on cell apoptosis and ROS production，as well as on TNF-α and PGE2 expression. Results　No toxic effect or proliferation influence of quercetin was observed on HGFs（P > 0.05）. There was a significant difference in overall cell migration rate among each group（F = 9.973，P < 0.05）. After 48 h of treatment，the group with quercetin at 20 μmol/L showed higher cell migration rate than the 10，50 μmol/L-quercetin treatment group and control group（P < 0.05）. Quercetin inhibited HGFs apoptosis stimulated by P.g LPS. Quercetin treatment could prevent and also inhibit the relative increase of ROS in HGFs induced by P.g LPS（P < 0.05）. Quercetin significantly inhibited the increased expression of TNF-α stimulated by P.g LPS（P < 0.05）；however，no significant difference in the expression of PGE2 was found between quercetin treatment group and control group（P > 0.05）. Conclusion　Quercetin at 20 μmol/L can promote in vitro HGFs cell migration and has anti-oxidative and anti-inflammatory protective effects.

Key words: quercetin；human gingival fibroblasts, HGFs；cytotoxicity；Porphyromonas gingivalis, P.g；lipopolysaccharide, LPS；inflammatory factors

摘要： 目的　研究槲皮素对牙龈卟啉单胞菌脂多糖（Porphyromonas gingivalis lipopolysaccharide，P.g LPS）刺激的人牙龈成纤维细胞（human gingival fibroblasts，HGFs）生物学行为的影响。方法　采用DNA片段凝胶电泳、CCK-8法、细胞划痕法观察不同浓度槲皮素（10、20、50、100 μmol/L）对体外培养HGFs的毒性作用，以及对HGFs增殖与迁移的影响。采用P.g LPS刺激HGFs来建立体外炎症刺激模型，通过流式细胞术、细胞活性氧（reactive oxygen species，ROS）检测、酶联免疫吸附试验（ELISA）和实时荧光定量PCR（qRT-PCR）进一步观察槲皮素对HGFs凋亡、ROS的产生及肿瘤坏死因子-α（tumor necrosis factor α，TNF-α）和前列腺素E2（prostaglandin E2，PGE2）表达的影响。结果　槲皮素对HGFs无毒性作用，且对HGFs的增殖无影响（P > 0.05）。各组细胞迁移率总的比较，差异有统计学意义（F = 9.973，P < 0.05），在处理48 h后，20 μmol/L槲皮素处理组细胞迁移率大于10、50 μmol/L槲皮素处理组以及对照组，差异均有统计学意义（均P < 0.05）。槲皮素对P.g LPS刺激的HGFs凋亡具有抑制作用，且其能够抑制并预防P.g LPS刺激的HGFs中ROS相对产生量增加现象（均P < 0.05）。槲皮素处理显著抑制了P.g LPS诱导的TNF-α表达增加现象（P < 0.05），而槲皮素处理组PGE2的表达水平与对照组比较，差异无统计学意义（P > 0.05）。结论　浓度为20 μmol/L的槲皮素能够促进体外培养HGFs的迁移，且具有抗氧化、抗炎保护作用。

关键词: 槲皮素, 人牙龈成纤维细胞, 细胞毒性, 牙龈卟啉单胞菌, 脂多糖, 炎症因子