Abstract:

Objective    To investigate the effect of different concentrations of glucose on human gingival fibroblasts secreting collagenⅠ，collagen Ⅲ and fibronectin.  Methods    The human gingival fibroblasts were cultured in different concentrations of glucose solution for 3 days to 7 days respectively，the concentrations being 5.5，7.0，8.8，10.0，15.0 and 20.0 mmol/L. The amount of collagenⅠ，collagen Ⅲ and fibronectin secreted by the human gingival fibroblasts were measured through Elisa method. Results    When the human gingival fibroblasts were cultured for 3 days，the amount of COL1 decreased in the glucose solution whose concentration was higher than 10.0 mmol/L；the amount of COL3 decreased when the glucose concentration was higher than 15.0 mmol/L，and so was FN. When the human gingival fibroblasts were cultured for 7 days，the amount of COL1，COL3 and FN decreased in the glucose solution whose concentration was higher than 10.0 mmol/L. Conclusion    With glucose concentrations increasing，the amount of collagenⅠ，collagen Ⅲ and fibronectin decreases，and the decrease is more significant in the high concentration of glucose solution.

Key words: glucose；concentration；human gingival fibroblasts；collagen；fibronectin, FN

摘要：

目的    探讨不同浓度葡萄糖对人牙龈成纤维细胞分泌Ⅰ（COL1）、Ⅲ型胶原蛋白（COL3）及纤维粘连蛋白（FN）的影响。方法    分别于5.5、7.0、8.8、10.0、15.0、20.0 mmol/L的葡萄糖溶液中培养人牙龈成纤维细胞3 ~ 7 d，酶联免疫吸附试验（ELISA）法测定其分泌COL1、COL3及FN的量。结果    人牙龈成纤维细胞培养3 d后，葡萄糖浓度高于10.0 mmol/L时，COL1的分泌量减少；高于15.0 mmol/L时，COL3的分泌量减少；FN与COL3情况相似。培养7 d后，葡萄糖浓度高于10.0 mmol/L时，COL1、COL3及FN的分泌量均减少。结论    随着葡萄糖浓度的增高，人牙龈成纤维细胞分泌COL1、COL3及FN的量均有减少趋势，且高糖条件下更为明显。

关键词: 葡萄糖, 浓度, 人牙龈成纤维细胞, 胶原蛋白, 纤维粘连蛋白