Abstract:

Abstract：Objective To study the expression of lymphoid enhancer factor-1 （LEF-1） mRNA in the human periodontal membrane cells under the action of fluid shear stress. Methods Human periodontal membrane cells were primarily cultured. The human periodontal membrane cells suffered from the fluid shear stress at 1.2 Pa for 0 h，0.5 h，2 h，4 h and 8 h by fluid shear loading system，then RT-PCR was used to test the expression of LEF-1 mRNA of cells before and after human periodontal membrane cells were stressed. Results The human periodontal membrane cells were stressed at 1.2 Pa for 8h by the fluid shear stress and the expression of LEF-1 mRNA increased. Conclusion The stimulation of fluid shear stress can activate the transcription of LEF-1 in human periodontal membrane cells. The reason of the expression increase of LEF-1 mRNA may be the response of Wnt signal pathway under the action of fluid shear stress.

Key words: periodontal membrane cells, LEF-1 mRNA, fluid shear stress

摘要：

目的 探讨人牙周膜细胞在流体剪切力作用下淋巴样增强因子-1（lymphoid enhancer factor-1，LEF-1）mRNA的表达。方法 对人牙周膜细胞进行原代培养；通过流体剪切力加力系统对人牙周膜细胞施加1.2 Pa的流体剪切力，作用0 、0.5、2、4、8 h后，用RT-PCR方法检测细胞受力前后LEF-1 mRNA的表达变化。结果 人牙周膜细胞在1.2 Pa的流体剪切力作用8 h后，LEF-1 mRNA的表达升高。结论 流体剪切力刺激活化了人牙周膜细胞中LEF-1的转录，LEF-1 mRNA的表达升高，可能是在剪切应力作用下Wnt信号通路的应答反应。

关键词: 牙周膜细胞, LEF-1 mRNA, 流体剪切力