Abstract:

Abstract： Objective To investigate MMP-2 and TIMP-2 in gingival crevicular fluid of replanted avulsed incisors，and to study the difference at different stages after replantation. Methods Totally twenty replanted avulsed teeth from children aged 7 to 13 were chosen randomly； replant and splint the avulsed teeth according to guidelines for the management of traumatic dental injuries.Ⅱ. Avulsion of permanent teeth， International Dental Truman Association.MMP-2 and TIMP-2 concentration was measured respectively at the first， third and seventh day after replantation；the difference among three stages were compared. MMP-2∶TIMP-2 ratio was also compared among three stages. Results MMP-2 and TIMP-2 concentration at first day was significantly higher than those of third and seventh day；the MMP-2∶TIMP-2 ratio at first day was significantly higher than that at third and seventh day. Conclusion MMP-2 and TIMP-2 concentration in replanted avulsed upper incisor dramatically changes in the early stage after replantation， indicting that MMP-2 and TIMP-2 may play an important role in reconstruction of periodontal tissues.

Key words: avulsed tooth, replantation, gingival crevicular fluid, MMP-2, TIMP-2

摘要：

目的 对脱位牙再植术后龈沟液（gingival cervicular fluid， GCF）内基质金属蛋白酶（matrix metalloproteinase，MMP）-2和基质金属蛋白酶组织抑制因子（tissue inhibitor of metalloproteinase，TIMP）-2的含量进行定量分析，并研究术后不同时间段其变化规律，探讨GCF内MMP-2及TIMP-2在牙周组织改建中的作用。方法 选择2010年8月至2012年3月在温州医科大学附属口腔医院就诊的20例7～13岁上颌中切牙外伤脱位患儿，参照国际牙外伤协会的年轻恒牙外伤管理指南2（2007年版）中的处理步骤行脱位牙再植术并进行固定，分别于术后第1、3、7天采集再植牙的GCF并定量测定其中MMP-2及TIMP-2的含量，比较不同时间段GCF内MMP-2及TIMP-2含量的差别，并比较MMP-2与TIMP-2比值（MMP-2/TIMP-2）的差别。结果 术后第1天GCF内MMP-2及TIMP-2的含量均显著高于第3天和第7天，且术后第1天两者比值亦显著高于第3和第7天。结论 脱位牙再植术后前期阶段（1～3 d）GCF中MMP-2及TIMP-2的含量变化剧烈，后期阶段（4～7 d）变化程度较小。提示MMP-2及TIMP-2可能在牙再植后牙周组织改建过程中扮演重要角色。

关键词: 脱位牙, 再植术, 龈沟液, 基质金属蛋白酶-2, 基质金属蛋白酶组织抑制因子-2