中国实用口腔科杂志

中国实用口腔科杂志

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声动力疗法辅助治疗大鼠牙周炎效果研究

栾小敏，庄德舒，代佳音，路海艳，毕良佳   

  1. 哈尔滨医科大学附属第四医院口腔科，黑龙江  哈尔滨  150001
  • 出版日期:2018-02-15 发布日期:2018-03-13
  • 基金资助:

    国家自然科学基金（81670994）

  • Online:2018-02-15 Published:2018-03-13

摘要：

目的    研究血卟啉单甲醚（HMME）介导的声动力疗法（SDT）辅助治疗大鼠牙周炎的治疗效果，为SDT应用于牙周炎临床治疗提供理论基础及实验依据。方法    通过上颌第一磨牙牙颈部丝线结扎诱导建立54只Wistar大鼠牙周炎模型。6周后，将建模成功的54只大鼠随机分为3组，每组18只。（1）对照组：生理盐水冲洗大鼠上颌第一磨牙牙周袋；（2）单纯龈下刮治和根面平整（SRP）组：大鼠上颌第一磨牙行SRP治疗后，生理盐水冲洗；（3）SRP联合SDT组：大鼠上颌第一磨牙行SRP治疗，生理盐水冲洗，颊侧牙龈局部注射1 mL HMME（40 μg/mL）避光孵育90 min后，联合频率1 MHz 、强度3 W/cm2的超声治疗 10 min。SDT隔天治疗1次，治疗后6、10、14 d每组分别处死6只大鼠，取上颌标本，行组织病理学检测及免疫组化分析。结果    SRP联合SDT组较对照组及单纯SRP组炎症细胞显著减少，免疫炎症因子核因子κB 受体活化因子配体（RANKL）表达呈弱阳性，抗酒石酸酸性磷酸酶（TRAP）阳性细胞明显减少，差异有统计学意义（P＜0.05）。结论    SDT辅助治疗大鼠牙周炎效果显著。

关键词: 牙周炎, 声动力疗法, 血卟啉单甲醚, 洁治和刮治

Abstract:

Objective    To evaluate the effect of HMME-mediated adjunctive sonodynamic therapy（SDT）on periodontitis in rats，in order to provide theoretical and experimental evidence for the clinical application of SDT. Methods    Ligatures were placed on the subgingival regions of the first maxillary molar in 54 rats to induce the periodontitis models. Then the rats were randomly divided into three groups with 18 rats in each group.（1）The control group was treated by normal saline（NS） to wash the periodontal pocket；（2）the scaling and root planing（SRP）group received SRP and was washed with normal saline；（3）the SRP plus SDT group was treated by SRP and washed with normal saline， and then was treated through subcutaneous injection of HMME 1 mL（40 μg/mL）. After 90 min of incubation in the dark，the ultrasonic treatment at the frequency of 1 MHz and the intensity of 3W/cm2 was given for 10 minutes. SDT was performed every other day. Six rats from each group were sacrificed at 6，10，or 14 days postoperatively and periodontal tissue samples were taken for histological examination and immunohistochemical analysis. Results    SRP plus SDT group had reduced number of inflammatory cells. The expression of receptor activator of nuclear factor kappa-B ligand （RANKL）was weakly positive，and the number of positive Tartrate-resistant acid phosphatase（TRAP） was significantly reduced （P < 0.05），compared to the SRP and the control group（P < 0.05）. Conclusion    SDT is an effective adjunctive therapy for periodontitis in rats.

Key words: periodontitis, sonodynamic therapy, hematoporphyrin monomethyl ether, scaling