中国实用口腔科杂志

中国实用口腔科杂志

• 论著 • 上一篇    下一篇

四碘荧光素钠对口腔龈上菌斑抑制效果研究

李若珍1，吕永昌1，毕良佳2   

  1. 1. 哈尔滨市第一医院口腔科，哈尔滨  150010；2. 哈尔滨医科大学附属第四医院口腔医疗中心，哈尔滨  150001
  • 出版日期:2016-01-15 发布日期:2016-02-26

  • Online:2016-01-15 Published:2016-02-26

摘要：

目的    探讨不同质量浓度四碘荧光素钠对口腔龈上菌斑的细菌抑制效果。方法    选择2015年3—6月来哈尔滨市第一医院口腔科就诊的牙周炎患者5例，收集其口腔龈上菌斑，溶于无菌生理盐水中，离心取菌悬液，比浊法配成标准浓度。在避光条件下，将菌悬液分别与不同质量浓度的四碘荧光素钠溶液（以无菌生理盐水为空白对照）混合，孵育培养后计数平板菌落，观察不同质量浓度四碘荧光素钠的细菌抑制效果。结果    质量浓度为0.5、1.0、2.0、4.0、8.0 g/L的四碘荧光素钠平板上的菌落数分别为303 ± 39.09、255 ± 51.18、111 ± 19.32、115 ± 28.54、121 ± 39.80，与空白对照平板上的菌落数（374 ± 14.83）比较，差异均有统计学意义（P < 0.05）。当四碘荧光素钠质量浓度为0.5 ~ 2.0 g/L时，随着其质量浓度的增加，细菌失活率显著增高；在质量浓度为2.0 g/L时，细菌失活率达到70%左右；质量浓度为4.0 ~ 8.0 g/L时，细菌失活率略下降但变化幅度不明显。结论    四碘荧光素钠对口腔龈上菌斑有抑制作用。

关键词: 龈上菌斑, 四碘荧光素钠, 抑菌作用

Abstract:

Objective    To study the antibacterial effect of different concentration of Erythrosin B on oral supragingival plaque. Methods    Oral supragingival plaque was collected from 5 patients with periodontitis，diagnosed by the Department of Stomatology，the First Hospital of Harbin City. The suspensions were configured at the standard concentration with Turbidimetry for use. The configured suspensions were mixed with the Erythrosin B solutions respectively at the different concentrations in the condition of dark. The final bacterial suspensions were incubated and cultured. Photograph and count each plate. Compare the bacterial colony numbers between the blank group and experimental groups and the results were analyzed statistically. Results    When the Erythrosin B concentration was 0.5，1.0，2.0，4.0 and 8.0 g/L，the bacterial colony numbers in experimental groups （303 ± 39.09，255 ± 51.18，111 ± 19.32，115 ± 28.54 and 121 ± 39.80）showed differences compared with that of the blank group（374 ± 14.83）（P < 0.05）. The bacterial inactivation rate grew significantly with the increase of concentration when it was between 0.5 g/L and 2.0 g/L （P < 0.05）. The bacterial inactivation was about 70% when the concentration was 2.0 g/L. The growth of bacterial inactivation rate decreased with the concentration when it was between 4.0 g/L and 8.0 g/L，but there was no significant differences（P > 0.05）. Conclusion        Erythrosin B has antibacterial effect on oral supragingival plaque.

Key words: supragingival plaque, Erythrosin B, antibacterial effect