中国实用口腔科杂志

中国实用口腔科杂志

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氯化锂对老龄大鼠口腔颌面部外科术后认知功能影响研究

张伦强，李书涛，何晓帆，金玄玉，张    倩   

  1. 中国医科大学附属口腔医院麻醉科，沈阳  110002
  • 出版日期:2015-05-15 发布日期:2015-07-22
  • 通讯作者: 张倩
  • 基金资助:

    辽宁省自然科学基金项目（201202256）

  • Online:2015-05-15 Published:2015-07-22

摘要：

目的    观察氯化锂预处理对老龄大鼠口腔颌面外科术后认知能力的影响。方法    本研究于2013年6月至2014年1月在中国医科大学药学院实验室及口腔医院中心实验室进行。老龄雄性SD大鼠48只随机分为3组，每组16只，包括正常对照组（C组）、手术麻醉组（O组）、氯化锂预处理组（L组）。每组大鼠再随机分为2组：8只用于术后3 d Morris水迷宫试验，检测其行为学特征；另外8只于术后24 h行断头处理，同时提取脑组织并分离海马组织。用ELISA和Western Blotting法检测海马内白细胞介素-1β（IL-1β）、糖原合成酶激酶-3β（GSK-3β）、磷酸化糖原合成酶激酶-3β［（p-GSK-3β（ser9）］的表达。结果    Morris水迷宫试验表明，术后第1天L组及O组都较C组潜伏期明显延长，游泳距离也较C组长，L组潜伏期及游泳距离都较O组缩短，差异均有统计学意义（均P＜0.05）；术后第2天L组及O组均较第1天稍有缩短，术后第3天较第2天也有所改善。大鼠对空间探索实验表明：C组较L组和O组在平台所在象限停留的时间明显延长、穿越平台的频率也增多。另外，L组及O组IL-β含量都较C组高，差异有统计学意义（均P＜0.05），L组明显低于O组，差异有统计学意义（P＜0.05）。3组GSK-3β差异无统计学意义（均P＞0.05）。L组及O组p-GSK-3β（ser9）含量较C组显著降低；L组较O组含量高，差异有统计学意义（P < 0.05）。结论    氯化锂预处理对老龄大鼠术后炎性因子表达有抑制作用，同时也能增加海马内GSK-3β磷酸化，使p-GSK-3β（ser9）表达上调，抑制脑细胞凋亡，从而改善口腔颌面外科手术后认知能力。

关键词: 氯化锂, 口腔颌面外科, 认知功能障碍

Abstract:

Objective    To observe the effects of lithium chloride pretreatment on cognitive ability of aged rats after oral and maxillofacial surgery. Methods    A total of 48 aged male SD rats（18 ~ 20 months old，weight 550 ~ 700 g） were bought from the Experimental Animal Center of China Medical University . These rats were randomly divided into three groups，including the normal control group（group C，n=16），surgery and anesthesia group（group O，n=16），and lithium chloride preconditioning group（group L，n=16）.Rats in each group were randomly divided into two parts，one part was given Morris water maze test three days after the surgery and its characteristics of behavior tested. Another part was decapitated 24 h after the surgery and extracted and the hippocampus brain separated at the same time. Test expression content of IL-1β GSK-3β p-GSK-3β（ser9）in the hippocampus by Elisa and Western blotting detection method respectively. Results    Morris water maze test showed that：the first day after surgery，latencies of group L and O were significantly longer than group C；compared with group C in swimming distance， latency and swim distances of group L were shorter than group O. With the comparison of multiple analysis of variance，differences were statistically significant（P＜0.05）. Groups L and O in the second day were slightly shortened compared to the first day，on the third day after surgery it has also improved over the second day. Space exploration experiments in rats showed that：the dwell time of group C was significantly longer in the platform quadrant and the frequency of crossing the platform also increased compared with groups L and O. In addition，group L and group O were higher in IL-β levels than group C，the difference being statistically significant by analysis of variance（P＜0.05），but group L was significantly lower compared with group O，the difference being statistically significant（P＜0.05）. The content of GSK-3β of three groups were of no significant difference（P > 0.05），but p-GSK-3β（ser9）content was significantly lower in group L and O than in group C. The content of GSK-3β of group L was higher than in group O，the difference being statistically significant（P＜0.05）. Conclusion    Pretreatment with lithium chloride in postoperative aged rats can inhibit the expression of inflammatory cytokines and increase GSK-3β phosphorylation in the hippocampus cells，so p-GSK-3β（ser9）upregulates and inhibites  the apoptosis of brain cells，thereby improved cognitive abilities after the cavity and maxillofacial surgery.

Key words: lithium chloride, oral and maxillofacial surgery, cognitive dysfunction