关键词: 组织工程, 脱细胞真皮基质支架, 人口腔黏膜, 移植

Abstract:

Objective　To investigate the role of grafted tissue-engineered human oral mucosa in the wound healing of nude mice. Methods　This study was conducted at the Animal Experimental Center of Institute of Basic Medicine of Chinese Academy of Medical Sciences from November 2016 to December 2016. Select 8 healthy nude mices，male，aged 3 to 4 weeks，weighing 14 ~ 15 g. Animals were anesthetized with 0.8% pentobarbital sodium. A 1.0 cm×1.0 cm full-thickness skin wound was created in the neck and back region in every mice，leaving the fascia intact. Tissue-engineered human oral mucosa was placed on the fascia of the neck and fixed in place with suture，and then fixed with a bolster dressing. In the control group，acellular dermis matrix（ADM）was placed on the fascia of the back，and all other surgical manipulation was the same as in the tissue-engineered human oral mucosa group. Eight days after grafting with the tissue-engineered human oral mucosa and ADM，the bolster dressings were removed. Histopathologic examination of the skin flaps was performed by HE staining. Results　Histopathological analysis of tissue-engineered human oral mucosa graft revealed a well-developed，multilayered and stratified squamous epithelium，and resembled native skin. But a portion of the tissue-engineered human oral mucosa epithelial layer peeled away from the ADM and ADM began to degrade. In contrast，no epithelial coverage of the ADM observed for 8 days after grafting. Conclusion　Wound healing can be enhanced by using tissue-engineered human oral mucosa graft.

Key words: tissue engineering, acellular dermis matrix scaffold, human oral mucosa, graft