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IgA肾病外周血单个核细胞T-betGATA-3ROR-γt和Foxp3mRNA的表达及其意义研究

方明徐颢王可平刘越坚陈淑妮林洪丽   

  1. 作者单位:大连医科大学附属第一医院肾内科,辽宁 大连 116011
  • 出版日期:2014-09-01 发布日期:2014-09-05
  • 通讯作者: 林洪丽
  • 基金资助:

    卫生部国家临床重点专科(肾病科)建设项目(2012年);辽宁省医学高峰建设工程项目(2011年)

T-bet,GATA-3,ROR-γt and Foxp3 mRNA levels of helper T cells in peripheral blood mononuclear cells from patients with IgA nephropathy.

FANG MingXU HaoWANG Ke-pingLIU Yue-JianCHEN Shu-NiLIN Hong-li.   

  1. Department of Nephrology,the First Affiliated Hospital of Dalian Medical University,Dalian 116011,China
  • Online:2014-09-01 Published:2014-09-05

摘要:

目的 在mRNA水平探讨IgA肾病患者外周血Th1、Th2、Th17和Treg细胞免疫失衡及其与IgA肾病(IgAN)牛津分型的相关性。方法 以2010年11月至2011年11月在大连医科大学附属第一医院肾内科经肾活检病理确诊的原发性IgAN患者为研究对象,应用实时荧光定量PCR检测IgAN患者(20例)和健康对照组(10名)外周血单个核细胞的特异性转录因子T-bet、GATA-3、ROR-γt、Foxp3的mRNA表达水平。结果 与对照组比较,IgAN组ROR-γt表达明显升高(P<0.05)、Foxp3表达明显下降(P<0.05),且ROR-γt/Foxp3比值明显升高(P<0.05);而T-bet、GATA-3的表达与对照组相比差异无统计学意义(P>0.05)。按IgAN牛津分型病理指标分组结果比较显示,M1组T-bet表达及T-bet/GATA-3的比值较M0组明显升高(P<0.05)。进一步相关分析显示,IgAN组T-bet表达与系膜增殖积分呈显著正相关(r=0.541,P<0.01)。S1组T-bet表达显著升高(P<0.01),ROR-γt表达无明显差异(P>0.05),T-bet/ROR-γt比值明显升高(P<0.05);而GATA-3、Foxp3无变化(P>0.05)。结论 IgAN患者外周血单个核细胞在mRNA水平出现了偏向Th17极化,而Treg的应答被抑制。IgAN系膜增殖程度与Th1极化呈正相关,而肾小球节段硬化的Th1/Th17的比值明显升高。

关键词: IgA肾病, 辅助性T细胞, 转录因子

Abstract:

Abstract:Objective To investigate the T-bet,GATA-3,ROR-γt and Foxp3 mRNA expression levels in peripheral blood mononuclear cells (PBMCs) from patients with IgA nephropathy and their correlations with Oxford Classification of IgA nephropathy.Methods Twenty patients with IgA nephropathy diagnosed in our hospital from November 2010 to 2011 and ten healthy volunteers were included in this study.Real time quantitative polymerase chain reaction (RT-PCR) was used to detect the T-bet,GATA-3,ROR-γt and Foxp3 mRNA expression levels in PBMCs from the two groups.Results ROR-γt mRNA expression levels and the ratio of ROR-γt/Foxp3 in patients with IgA nephropathy were higher while Foxp3 mRNA levels were lower than those in the controls (both P<0.05).According by Oxford Classification of IgA nephropathy,T-bet mRNA expression levels and the ratio of T-bet/GATA-3 in the M1 group were high than those in the M0 group (P<0.05),and correlation analysis showed that T-bet mRNA expression was associated with mesangial hypercellularity score (r=0.541,P<0.01).T-bet mRNA expression levels and the ratio of T-bet/ROR-γt in the S1 group were higher than those in the S0 group (P<0.05).No significantly differences were detected in GATA-3 and Foxp3 mRNA expression levels between in the S0 and S1 groups (P>0.05).Conclusion There are polarization of Th1 and Th17 in patients with IgA nephropathy.The Treg response is inhibited.Th1 polarization is positively correlated with mesangial hypercellularity score.The ratio of Th1/Th17 is elevated in IgA nephropathy patients in whom segmental glomerulosclerosis exists.

Key words: IgA nephropathy, helper T cells, transcriptional factors

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