中国实用口腔科杂志

中国实用口腔科杂志

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槟榔碱对Hacat细胞上皮模型通透性的影响及钙离子的调节作用研究

陈蓉1 ，侯冬兰1  ，尹晓敏2  ，高义军1   

  1. 1.中南大学湘雅二医院口腔医疗中心，湖南  长沙 410011；2. 中南大学湘雅医院口腔医学中心，湖南  长沙  410008
  • 出版日期:2017-12-15 发布日期:2018-01-09
  • 基金资助:

    湖南省自然科学基金资助项目（14JJ2030）

  • Online:2017-12-15 Published:2018-01-09

摘要：

目的    研究槟榔碱对Hacat细胞上皮模型通透性的影响及Ca2+对其的调节作用。方法    建立Hacat细胞上皮体外模型；设置对照组（A组）、槟榔碱处理组（B组）及Ca2++槟榔碱处理组（C组），以荧光黄表观通透系数（Papp）评价槟榔碱及Ca2+对细胞模型通透性的影响。结果    在B组，各小组荧光黄Papp均大于相应A组（P < 0.05），且荧光黄Papp随槟榔碱浓度及干预时间增加而增大（P < 0.05）。在C组，各小组荧光黄Papp分别比相同浓度和时间点的B组低（P < 0.05），各小组荧光黄Papp均大于A组（P < 0.05），且荧光黄Papp随槟榔碱浓度及干预时间增加而增大（P < 0.05）。结论    槟榔碱导致上皮屏障通透性增大，可能是口腔黏膜下纤维性变（oral submucous fibrosis，OSF）发病作用机制之一。Ca2+预处理使槟榔碱增加上皮通透性的作用减弱。

关键词: 口腔黏膜下纤维性变, 槟榔碱, 钙离子, 通透性, Hacat细胞

Abstract:

Objective    To study the effect of arecoline on the permeability of Hacat cell epithelia model in vitro and the adjustment of Ca2+ on it. Methods    Establish  the Hacat cell epithelia monolayer model in vitro by culturing Hacat cell. The models were divided into control group （group A），arecoline treatment group （group B） and “calcium+ arecoline” treatment group （group C）. The values of Lucifer Yellow Papp were used to estimate the permeability changes of models after treatment with arecoline and calcium. Results    In group B，the Lucifer Yellow Papp of each subgroup was higher than that of group A （P < 0.05） and the Lucifer Yellow Papp increased as the arecoline concentration and treatment time were increased （P < 0.05）. In group C，after pretreatment with calcium，the Lucifer Yellow Papp of each subgroup were lower than that of group B at corresponding concentration and time points （P < 0.05）；while the Pappof each subgroup was higher than that of group A（P < 0.05）. Besides，the Lucifer Yellow Papp increased as the arecoline concentration and treating time were increased （P < 0.05）. Conclusion      The increase of permeability of oral epithelia model by arecoline may play an important role in the pathogenesis and development of oral submucous fibrosis. The pretreatment with calcium，to some extent，can weaken the effect of arecoline on the permeability of Hacat cell monolayer model.

Key words: oral submucous fibrosis, OSF；arecoline；calcium ion；permeability；Hacat cells