关键词: 纤维蛋白原, 中性多形核白细胞, 促炎细胞因子

Abstract: Effect of different forms of fibrinogen on the secretion of IL-1β and IL -8 by human peripheral polymorphonuclear leukocytes. GE Song*， WU Ya-fei， LIU Tian-jia， ZHAO Lei， MENG Shu.* West China School of Stomatology， Sichuan University， Chengdu 610041， China
Abstract: Objective To study the effect of different forms of fibrinogen on the secretion of IL-1β and -8 of human peripheral polymorphonuclear leukocytes （PMNs） and its pathological role in the pathogenesis of periodontitis. Methods Peripheral PMNs were isolated by discontinuous density gradient centrifuging technique. The freshly isolated PMNs were cultured in the present of different forms of fibrinogen which existed as soluble form or immobilized form， and the levels of IL-1β and -8 in the supernatants produced by PMNs were measured by ELISA technique. Results The differences in the levels of IL-1β and -8 released by peripheral PMNs between the two groups after PMNs interacted with immobilized and soluble fibrinogen， were significant（P ＜ 0.05）. Depending on the interaction with immobilized fibrinogen， but not soluble fibrinogen， PMNs secreted significantly higher levels of IL-1β and IL-8. Conclusion The interaction between PMNs and immobilized fibrinogen could induce PMNs to release higher levels of pro-inflammatory cytokines， IL-1β and IL-8， which may be involved in the pathogenesis of periodontitis.

Key words: fibrinogen, polymorphonuclear leukocytes, pro-inflammatory cytokines