中国实用口腔科杂志

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口腔鳞状细胞癌C-erbB-2蛋白表达 基因扩增及其临床意义

邓巍谢洪孙晓菊   

  1. 作者单位:辽宁省人民医院口腔科,沈阳110016
  • 出版日期:2014-05-15 发布日期:2014-05-23
  • 通讯作者: 孙晓菊

The expression of C-erbB-2 protein,gene amplification and its clinical significance in oral squamous cell carcinoma(OSCC).

DENG WeiXIE HongSUN Xiao-ju   

  1. People's Hospital of Liaoning Province,Shengyang 100016,China
  • Online:2014-05-15 Published:2014-05-23

摘要:

目的 探讨C-erbB-2在口腔鳞状细胞癌(OSCC)中的蛋白表达及基因扩增,并评估其在OSCC发生、发展和预后中的价值。方法 收集2003年1月至2013年6月辽宁省人民医院手术切除并经病理确诊为OSCC的石蜡包埋组织蜡块60例及正常口腔黏膜组织蜡块30例,分别采用免疫组织化学SP法与荧光原位杂交(FISH)技术检测C-erbB-2蛋白表达及基因扩增。结果 C-erbB-2蛋白在OSCC、正常口腔黏膜组织中的表达阳性率分别为31.7%、3.3%;扩增阳性率分别为28.3%、0;OSCC中C-erbB-2蛋白表达、基因扩增与正常口腔黏膜组织相比较,差异具有统计学意义(P < 0.05);在OSCC组织中C-erbB-2蛋白表达、基因扩增与患者的年龄、性别、肿瘤大小、分化程度均无相关性(均P > 0.05),与肿瘤淋巴结转移、远处转移、临床分期相关(χ2 = 10.808、17.100、4.627,χ2 = 8.840、20.044、5.102,均P < 0.05)。结论 C-erbB-2与OSCC的发生、发展有关,可以将其作为判断OSCC的生物学行为的重要参考指标。

关键词: 口腔鳞状细胞癌, C-erbB-2, 免疫组织化学, 荧光

Abstract:

Abstract:Objective To investigate the expression of C-erbB-2 protein and gene amplification in OSCC and to evaluate C-erbB-2 in OSCC occurrence,development and prognosis. Methods Use immunohistochemical SP method and in situ chemical hybridization (FISH)to detect expression of C-erbB-2 protein and gene amplification in 60 cases of OSCC and 30 cases of normal oral mucosa in paraffin-embedded tissue chip material. Results The positive rate of C-erbB-2 expression in OSCC and normal oral mucosa tissues was 31.7% and 3.33% respectively. The positive rate of C-erbB-2 gene amplification was 28.3% and 0.There were significant differences between OSCC and oral mucosa tissues in the expression and amplification of C-erbB-2.The expression and amplification of C-erbB-2 in OSCC had no correlation with age,gender or tumor differentiation degree of patients(P > 0.05),but were correlated with the lymph node metastasis,distant metastasis and clinical stage(P < 0.05).Conclusion C-erbB-2 is closely related to the occurrence and development of OSCC,and can be considered as an important index for determing the biological behavior of OSCC.

Key words: OSCC, C-erbB-2, immunohistochemistry, fluorescence

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